US 11,867,671 B2
Method for amino acid analysis and system for amino acid analysis
Masami Shibukawa, Saitama (JP)
Assigned to NATIONAL UNIVERSITY CORPORATION SAITAMA UNIVERSITY, Saitama (JP); and HITACHI HIGH-TECH SCIENCE CORPORATION, Tokyo (JP)
Filed by National University Corporation Saitama University, Saitama (JP); and Hitachi High-Tech Science Corporation, Tokyo (JP)
Filed on Dec. 19, 2019, as Appl. No. 16/721,185.
Claims priority of application No. 2018-241936 (JP), filed on Dec. 26, 2018.
Prior Publication US 2020/0217826 A1, Jul. 9, 2020
Int. Cl. G01N 30/54 (2006.01); G01N 30/52 (2006.01)
CPC G01N 30/54 (2013.01) [G01N 2030/522 (2013.01)] 6 Claims
OG exemplary drawing
 
6. A system for separating a basic amino acid from a non-basic amino acid in a mixture of a plurality of amino acids comprising the basic amino acid and the non-basic amino acid, the system comprising:
an eluent having a composition that comprises: a polybasic acid, a cation source, and water, having a pH in a range of 5.0 or lower at a temperature of 25° C., and being only one eluent present in the system and having a same composition therein;
a separation column packed with a cation exchange resin and configured so that a sample comprising the mixture of the plurality of amino acids passes through the separation column with the eluent, thereby the basic amino acid and the non-basic amino acid being eluted separately;
a heater configured to heat the separation column by applying a temperature gradient including a temperature region of 100° C. or higher;
a pressurizer configured to flow the sample together with the eluent through the separation column by adding pressure to the separation column; and
a detector configured to detect each of the separately eluted amino acids from the separation column,
wherein the cation source is at least one salt of an alkali metal selected from the group consisting of a lithium (Li) salt and a sodium (Na) salt.