US 11,867,614 B2
Method for determining the quality of an animal's semen
Carlo Bertozzi, Sombreffe (BE); Emilie Henrotte, Bonneville (BE); Christophe Boccart, Ciney (BE); Vincent Baeten, Hoeilaart (BE); and Frédéric Dehareng, Namur (BE)
Assigned to INOVEO, Ciney (BE)
Appl. No. 17/791,432
Filed by Inoveo, Ciney (BE)
PCT Filed Jan. 8, 2021, PCT No. PCT/EP2021/050234
§ 371(c)(1), (2) Date Jul. 7, 2022,
PCT Pub. No. WO2021/140175, PCT Pub. Date Jul. 15, 2021.
Claims priority of application No. 2020/5012 (BE), filed on Jan. 9, 2020.
Prior Publication US 2023/0042628 A1, Feb. 9, 2023
Int. Cl. G01N 21/31 (2006.01); A61D 19/02 (2006.01)
CPC G01N 21/31 (2013.01) [A61D 19/024 (2013.01)] 11 Claims
OG exemplary drawing
 
1. A method of determining the quality of an animal's semen, comprising the steps of:
collecting at least one fresh or frozen semen sample,
measuring at least one absorption spectrum Xj of at least one sample of said semen,
determining from said at least one absorption spectrum Xj, a value of the first derivative of the absorptions X′j,
calculating or more parameters, representative of the quality of said semen, selected from the group consisting of concentration Y1, mobility Y2, progressive spermatozoa rate Y3, viability Y4, percentage of live spermatozoa with stable phospholipids Y5, mitochondrial potential Y6, percentage of spermatozoa with peroxidized lipids Y7, percentage of spermatozoa with an intact acrosome Y8, the total antioxidant capacity TAC Y9, fatty acid composition Y10, percentage of spermatozoa having a normal morphology Y11, the osmolarity Y12 and glutathione GSH level Y13, non-return rate at 56 days Y14, the non-return rate at 90 days Y15, pregnancy diagnosis Y16, from said first derivative of the absorption X′j previously determined for the determining of the quality of said semen; wherein:
said concentration Y1 is calculated according to the mathematical law Y1=β0+Σj=1nβjX′j, where X′j(j∈[1;n]) is the first derivative of the absorption Xj, normalized by an SNV (“Standard Normal Variate”) pre-processing, for the wavenumber range [1,800 cm−1; 900 cm−1] and the weighting coefficients β0 and β(j∈[1;n]) are constants; and/or
said mobility Y2 is calculated according to the mathematical law Y2=β0+Σj=1nβjX′j, where X′j(j∈[1;n]) is the first derivative of the absorption Xj, normalized by an SNV pre-processing, for the wavenumber range [1,800 cm−1; 900 cm−1], and the weighting coefficients β0 and βj(j∈[1;n]) are constants; and/or
said progressive spermatozoa rate Y3 is calculated according to the mathematical law Y3=β0+Σj=1nβjX′j, where X′j(j∈[1;n]) is the first derivative of absorption Xj, normalized by an SNV pre-processing, for the wavenumber range [1,800 cm−1; 900 cm−1], and the weighting coefficients β0 and βj(j∈[1;n]) are constants; and/or
said viability Y4 is calculated according to the mathematical law Y4=β0+Σj=1nβjX′j, where X′j(j∈[1;n]) is the first derivative of the absorption Xj, normalized by an SNV pre-processing, for the wavenumber range [1,800 cm−1; 900 cm−1] and the weighting coefficients β0 and βj(j∈[1;n]) are constants; and/or
said percentage of live spermatozoa with stable phospholipids Y5 is calculated according to the mathematical law Y5=β0+Σj=1nβjX′j, where X′j(j∈[1;n]) is the first derivative of the absorption Xj, normalized by an SNV pre-processing, for the wavenumber range [1,800 cm−1; 900 cm−1] and the weighting coefficients β0 and βj(j∈[1;n]) are constants; and/or
said mitochondrial potential Y6 is calculated according to the mathematical law Y6=β0+Σj=1nβjX′j, where X′j(j∈[1;n]) is the first derivative of the absorption Xj, normalized by an SNV pre-processing, for the wavenumber range [1,800 cm−1; 900 cm−1] and the weighting coefficients β0 and βj(j∈[1;n]) are constants; and/or
said percentage of spermatozoa with peroxidized lipids Y7 is calculated according to the mathematical law Y7=β0+Σj=1nβjX′j, where X′j(j∈[1;n]) is the first derivative of the absorption Xj, normalized by an SNV pre-processing, for the wavenumber range [1,800 cm−1; 900 cm−1] and the weighting coefficients β0 and βj(j∈[1;n]) are constants; and/or
said percentage of spermatozoa with an intact acrosome Y8 is calculated according to the mathematical law Y8=β0+Σj=1nβjX′j, where X′j(j∈[1;n]) is the first derivative of the absorption Xj, normalized by an SNV pre-processing, for the wavenumber range [1,800 cm−1; 900 cm−1] and the weighting coefficients β0 and βj(j∈[1;n]) are constants; and/or
said total antioxidant capacity TAC Y9 is calculated according to the mathematical law Y9=β0+Σj=1nβjX′j, where X′j(j∈[1;n]) is the first derivative of the absorption Xj, normalized by an SNV pre-processing, for the wavenumber range [1,700 cm−1; 910 cm−1] and the weighting coefficients β0 and βj(j∈[1;n]) are constants; and/or
said fatty acid composition Y10 is calculated according to the mathematical law Y10=β0+Σj=1nβjX′j, where X′j(j∈[1;n]) is the first derivative of the absorption Xj, normalized by an SNV pre-processing, for the wavenumber range [1,800 cm−1; 900 cm−1] and for the wavenumber range [3,000 cm−1; 2,700 cm−1] and the weighting coefficients β0 and βj(j∈[1;n]) are constants; and/or
said morphology Y11 is calculated according to the mathematical law Y11=β0+Σj=1nβjX′j, where X′j(j∈[1;n]) is the first derivative of the absorption Xj, normalized by an SNV pre-processing, for the wavenumber range [1,800 cm−1; 900 cm−1] and the weighting coefficients β0 and βj(j∈[1;n]) are constants; and/or
said osmolarity Y12 is calculated according to the mathematical law Y12=β0+Σj=1nβjX′j, where X′j(j∈[1;n]) is the first derivative of the absorption Xj, normalized by an SNV pre-processing, for the wavenumber range [1,700 cm−1; 910 cm−1] and the weighting coefficients β0 and βj(j∈[1;n]) are constants; and/or
said GSH level Y13 is calculated according to the mathematical law Y13=β0+Σj=1nβjX′j, where X′j(j∈[1;n]) is the first derivative of the absorption Xj, normalized by an SNV pre-processing, for the wavenumber range [1,800 cm−1; 900 cm−1] and the weighting coefficients β0 and βj(j∈[1;n]) are constants; and/or
said non-return rate at 56 days Y14 is calculated according to the mathematical law Y14=β0+Σj=1nβjX′j, where X′j(j∈[1;n]) is the first derivative of the absorption Xj, normalized by an SNV pre-processing, for the wavenumber range [1,800 cm−1; 900 cm−1] and the weighting coefficients β0 and βj(j∈[1;n]) are constants; and/or
said non-return rate at 90 days Y15 is calculated according to the mathematical law Y15=β0+Σj=1nβjX′j, where X′j(j∈[1;n]) is the first derivative of the absorption Xj, normalized by an SNV pre-processing, for the wavenumber range [1,800 cm−1; 900 cm−1] and the weighting coefficients β0 and βj(j∈[1;n]) are constants; and/or
said pregnancy diagnosis Y16 is calculated according to the mathematical law Y16=β0+Σj=1nβjX′j, where X′j(j∈[1;n]) is the first derivative of the absorption Xj, normalized by an SNV pre-processing, for the wavenumber range [1,800 cm−1; 900 cm−1] and the weighting coefficients β0 and βj(j∈[1;n]) are constants.