	US 11,866,795 B2
	RNA separation and related techniques for determining viruses such as coronaviruses
Arnon Chait, Bay Village, OH (US); and Boris Y. Zaslavsky, Solon, OH (US)
Assigned to Analiza, Inc., Bay Village, OH (US)
Filed by Analiza, Inc., Bay Village, OH (US)
Filed on Nov. 16, 2022, as Appl. No. 17/987,973.
Application 17/987,973 is a continuation of application No. 17/695,994, filed on Mar. 16, 2022, granted, now 11,535,902.
Claims priority of provisional application 63/162,485, filed on Mar. 17, 2021.
Prior Publication US 2023/0151443 A1, May 18, 2023
Int. Cl. C12N 15/10 (2006.01); C12Q 1/68 (2018.01); C12Q 1/6809 (2018.01); C12Q 1/70 (2006.01); C12Q 1/6834 (2018.01); A61K 9/51 (2006.01); A61K 39/215 (2006.01); G01N 33/52 (2006.01); G01N 33/569 (2006.01)

CPC C12Q 1/701 (2013.01) [A61K 9/51 (2013.01); A61K 39/215 (2013.01); G01N 33/523 (2013.01); G01N 33/56983 (2013.01); G01N 2333/165 (2013.01)]

30 Claims

1. A method comprising:

partitioning a biological fluid comprising a virus and free nucleic acid in an aqueous multi-phase partitioning system, wherein at least 70% of the free nucleic acid partitions in a first phase of the partitioning system; and

determining the virus within the partitioning system using an agent comprising a signaling moiety and a virus-binding moiety, wherein the signaling moiety comprises gold nanoparticles.