	US 11,866,794 B2
	Cas-ready mouse embryonic stem cells and mice and uses thereof
Guochun Gong, Pleasantville, NY (US); Charleen Hunt, Montvale, NJ (US); Daisuke Kajimura, Sleepy Hollow, NY (US); Suzanne Hartford, Putnam Valley, NY (US); and Brian Zambrowicz, Sleepy Hollow, NY (US)
Assigned to Regeneron Pharmaceuticals, Inc., Tarrytown, NY (US)
Filed by Regeneron Pharmaceuticals, Inc., Tarrytown, NY (US)
Filed on Aug. 24, 2021, as Appl. No. 17/410,437.
Application 17/410,437 is a continuation of application No. 16/050,784, filed on Jul. 31, 2018, granted, now 11,130,999.
Claims priority of provisional application 62/539,275, filed on Jul. 31, 2017.
Prior Publication US 2021/0392862 A1, Dec. 23, 2021
This patent is subject to a terminal disclaimer.
Int. Cl. A01K 67/027 (2006.01); C12Q 1/6897 (2018.01); C12N 15/11 (2006.01); C12N 9/22 (2006.01); A61K 49/00 (2006.01); A61K 9/00 (2006.01); C12N 15/90 (2006.01)

CPC C12Q 1/6897 (2013.01) [A01K 67/027 (2013.01); A01K 67/0275 (2013.01); A61K 9/0019 (2013.01); A61K 49/0008 (2013.01); C12N 9/22 (2013.01); C12N 15/11 (2013.01); C12N 15/907 (2013.01); A01K 2217/072 (2013.01); A01K 2217/15 (2013.01); A01K 2217/206 (2013.01); A01K 2227/105 (2013.01); A01K 2267/03 (2013.01); A01K 2267/0393 (2013.01); C07K 2319/60 (2013.01); C12N 2310/20 (2017.05); C12N 2750/14141 (2013.01); C12N 2800/80 (2013.01); C12Q 2600/106 (2013.01)]

18 Claims

1. A mouse comprising in its germline a genomically integrated Cas9 expression cassette at a target genomic locus capable of expressing a gene,

wherein the target genomic locus is a Rosa26 locus,

wherein the Cas9 expression cassette comprises a coding sequence for a Cas9 protein,

wherein the Cas9 protein comprises the full sequence set forth in SEQ ID NO: 19, and

wherein the mouse expresses the Cas9 protein.