CPC C12Q 1/6872 (2013.01) [C12Q 2523/313 (2013.01)] | 20 Claims |
1. A mass spectrometry method for detecting a modified nucleobase in a nucleic acid species, comprising
a) providing a sample, wherein the sample comprises:
(i) a modified nucleobase attached in N-glycosidic linkage to a ribose or deoxyribose moiety of a nucleic acid, nucleoside, nucleotide or oligonucleotide; and
(ii) a matrix, wherein the matrix comprises at least one Brønsted acidic proton source; and
b) subjecting the sample to at least one laser pulse, wherein the laser fluence is about 70% to about 95% of the matrix storm level.
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