US 11,866,779 B2
Jettison-MS for nucleic acid species
Roger W. Giese, Hanover, MA (US); and Poguang Wang, Westborough, MA (US)
Assigned to Northeastern University, Boston, MA (US)
Appl. No. 16/961,074
Filed by Northeastern University, Boston, MA (US)
PCT Filed Feb. 28, 2019, PCT No. PCT/US2019/020103
§ 371(c)(1), (2) Date Jul. 9, 2020,
PCT Pub. No. WO2019/169163, PCT Pub. Date Sep. 6, 2019.
Claims priority of provisional application 62/636,685, filed on Feb. 28, 2018.
Prior Publication US 2021/0062257 A1, Mar. 4, 2021
Int. Cl. C12Q 1/68 (2018.01); C12Q 1/6872 (2018.01)
CPC C12Q 1/6872 (2013.01) [C12Q 2523/313 (2013.01)] 20 Claims
OG exemplary drawing
 
1. A mass spectrometry method for detecting a modified nucleobase in a nucleic acid species, comprising
a) providing a sample, wherein the sample comprises:
(i) a modified nucleobase attached in N-glycosidic linkage to a ribose or deoxyribose moiety of a nucleic acid, nucleoside, nucleotide or oligonucleotide; and
(ii) a matrix, wherein the matrix comprises at least one Brønsted acidic proton source; and
b) subjecting the sample to at least one laser pulse, wherein the laser fluence is about 70% to about 95% of the matrix storm level.