CPC C12Q 1/6862 (2013.01) [B82Y 5/00 (2013.01); C12Q 1/6844 (2013.01); C12Q 1/6853 (2013.01); C12Q 2545/101 (2013.01)] | 18 Claims |
1. A method for amplifying a molecular signal of an Input-Oligonucleotide, the method comprising
a) providing a plurality of Particle-A protected by oligonucleotide origami, wherein Particle-A is functionalized with a plurality of Oligonucleotide-1, wherein a sequence in Oligonucleotide 1 complementary to a sequence in a plurality of Oligonucleotide 2 hybridize to form Duplex 1-2, and sequence in Oligonucleotide 2 complementary to a sequence in oligonucleotide origami hybridize;
b) providing a plurality of Particle-B, wherein Particle-B is functionalized with a plurality of Oligonucleotide-3, wherein a sequence in Oligonucleotide-3 complementary to a sequence in a plurality of Stored-Input-Oligonucleotide hybridize to form Duplex 3-Y; and
c) mixing the Input-Oligonucleotide, plurality of origami protected Particle-A and plurality of Particle-B, in any order, wherein a cascade reaction occurs so that:
i) sequence in the Input-Oligonucleotide complementary to sequence in Oligonucleotide-2 hybridize to form Duplex Y-2, thereby removing Oligonucleotide-2 and the oligonucleotide origami, and exposing Oligonucleotide-1 on Particle A,
ii) sequence in Oligonucleotide-1 complementary to sequence in Oligonucleotide-3 hybridize to form Duplex 1-3, thereby releasing the Stored-Input-Oligonucleotide hybridized to the sequence in Oligonucleotide-3,
iii) sequence in the Stored-Input Oligonucleotide released in ii) complementary to sequence in Oligonucleotide-2 hybridize to form Duplex Y-2, thereby removing Oligonucleotide-2 and the oligonucleotide origami, and exposing Oligonucleotide-1 on Particle A to hybridize to complementary sequence in Oligonucleotide-3 to form Duplex 1-3, thereby releasing the Stored-Input-Oligonucleotide hybridized to the sequence in Oligonucleotide-3, and,
iv) where (iii) reoccurs until the cascade reaction terminates, when Particle A are fully deprotected.
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