US 11,866,771 B2
Emulsion chemistry for encapsulated droplets
Amy L. Hiddessen, Dublin, CA (US); and Benjamin J. Hindson, Livermore, CA (US)
Assigned to Bio-Rad Laboratories, Inc., Hercules, CA (US)
Filed by Bio-Rad Laboratories, Inc., Hercules, CA (US)
Filed on Jun. 11, 2021, as Appl. No. 17/345,834.
Application 17/345,834 is a continuation of application No. 16/447,315, filed on Jun. 20, 2019, granted, now 11,060,136.
Application 16/447,315 is a continuation of application No. 15/452,582, filed on Mar. 7, 2017, granted, now 10,378,048, issued on Aug. 13, 2019.
Application 15/452,582 is a continuation of application No. 12/976,827, filed on Dec. 22, 2010, granted, now 9,598,725, issued on Mar. 21, 2019.
Claims priority of provisional application 61/417,241, filed on Nov. 25, 2010.
Claims priority of provisional application 61/410,769, filed on Nov. 5, 2010.
Claims priority of provisional application 61/409,473, filed on Nov. 2, 2010.
Claims priority of provisional application 61/409,106, filed on Nov. 1, 2010.
Claims priority of provisional application 61/380,981, filed on Sep. 8, 2010.
Claims priority of provisional application 61/341,218, filed on Mar. 25, 2010.
Claims priority of provisional application 61/317,635, filed on Mar. 25, 2010.
Claims priority of provisional application 61/309,845, filed on Mar. 2, 2010.
Prior Publication US 2021/0363574 A1, Nov. 25, 2021
This patent is subject to a terminal disclaimer.
Int. Cl. C12Q 1/6848 (2018.01); C12Q 1/6844 (2018.01); C12Q 1/6816 (2018.01); C12Q 1/686 (2018.01)
CPC C12Q 1/6848 (2013.01) [C12Q 1/686 (2013.01); C12Q 1/6816 (2013.01); C12Q 1/6846 (2013.01)] 20 Claims
 
1. A method of generating skin-encapsulated capsules comprising:
a) providing an aqueous phase comprising:
i) at least one reagent;
ii) at least 0.01% by weight of a skin-forming protein;
b) forming an emulsion comprising droplets of said aqueous phase disposed in a continuous phase comprising an oil and an ionic surfactant;
c) heating said emulsion to at least 50° C. to create an interfacial skin between each droplet and the continuous phase, such that said droplets form skin-encapsulated capsules; and
d) adding a spacing fluid to the emulsion after heating the emulsion, wherein the spacing fluid is miscible with the continuous phase.