CPC C12Q 1/6837 (2013.01) [B01L 3/502715 (2013.01); C12Q 1/68 (2013.01); C12Q 1/6804 (2013.01); C12Q 1/6809 (2013.01); C12Q 1/6834 (2013.01); C12Q 1/6841 (2013.01); C12Q 1/6869 (2013.01); C12Q 1/6874 (2013.01); C12Q 1/6876 (2013.01); C40B 30/04 (2013.01); C40B 60/04 (2013.01); G01N 33/5308 (2013.01); G01N 33/543 (2013.01); G01N 33/54366 (2013.01); G01N 33/6845 (2013.01); C12Q 1/6858 (2013.01); C12Q 2600/156 (2013.01); C12Q 2600/158 (2013.01); G01N 2458/10 (2013.01)] | 24 Claims |
1. A system comprising:
a microcircuit arrangement and software configured to cause the microcircuit arrangement to associate a target nucleic acid on a substrate to a location in a tissue section; and
a plurality of probes contacted with the tissue section, wherein a probe of the plurality of probes comprises a capture agent, and wherein the capture agent comprises:
(i) a binding sequence configured to bind the target nucleic acid, or a portion thereof, of the tissue section, and
(ii) a coding tag sequence that corresponds to a location of the target nucleic acid in the tissue section,
wherein the software is further configured to cause the microcircuit arrangement to:
correlate the coding tag sequence, or a complement thereof, from its location on the substrate to its location in the tissue section; and
associate the coding tag sequence, or the complement thereof, to the target nucleic acid, or a complement thereof, configured to bind the binding sequence, thereby associating the target nucleic acid on the substrate to its location in the tissue section.
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