US 11,866,768 B2
Signal encoding and decoding in multiplexed biochemical assays
Emil P. Kartalov, Pasadena, CA (US); Aditya Rajagopal, Orange, CA (US); and Axel Scherer, Pasadena, CA (US)
Assigned to California Institute of Technology, Pasadena, CA (US)
Filed by California Institute of Technology, Pasadena, CA (US)
Filed on Jul. 23, 2020, as Appl. No. 16/937,464.
Application 16/937,464 is a continuation of application No. 15/914,356, filed on Mar. 7, 2018, granted, now 10,770,170.
Application 15/914,356 is a continuation of application No. 14/451,876, filed on Aug. 5, 2014, granted, now 10,068,051, issued on Sep. 4, 2018.
Application 14/451,876 is a continuation of application No. 13/756,760, filed on Feb. 1, 2013, granted, now 8,838,394, issued on Sep. 16, 2014.
Claims priority of provisional application 61/703,093, filed on Sep. 19, 2012.
Claims priority of provisional application 61/594,480, filed on Feb. 3, 2012.
Prior Publication US 2021/0012860 A1, Jan. 14, 2021
This patent is subject to a terminal disclaimer.
Int. Cl. G01N 33/48 (2006.01); G01N 33/50 (2006.01); C12Q 1/6825 (2018.01); G16B 25/00 (2019.01); G16B 5/00 (2019.01); C12Q 1/6851 (2018.01); G16B 25/20 (2019.01); G16B 40/10 (2019.01); C12Q 1/68 (2018.01); G01N 21/64 (2006.01); G06F 17/10 (2006.01); G06F 17/11 (2006.01); G16B 40/00 (2019.01)
CPC C12Q 1/6825 (2013.01) [C12Q 1/68 (2013.01); C12Q 1/6851 (2013.01); G01N 21/6486 (2013.01); G16B 5/00 (2019.02); G16B 25/00 (2019.02); G16B 25/20 (2019.02); G16B 40/10 (2019.02); G06F 17/10 (2013.01); G06F 17/11 (2013.01); G16B 40/00 (2019.02); Y02A 50/30 (2018.01)] 11 Claims
OG exemplary drawing
 
1. A method of unambiguously detecting any unique combination of presence or absence of a plurality of analytes, the method comprising:
(a) providing a sample from a subject comprising, or potentially comprising, said plurality of analytes,
(b) forming a mixture of said sample and hybridization probes, wherein at least one of said hybridization probes is configured to bind to at least one of said plurality of analytes corresponding with a respiratory viral pathogen, and wherein said hybridization probes further comprise at least one fluorophore and at most four fluorophores;
(c) exciting said at least one fluorophore to generate one or more signals if one or more of said plurality of analytes is present, wherein said one or more signals comprise at least one signal generated by excitement of said one or more fluorophores;
(d) measuring said one or more signals to generate a cumulative intensity measurement, wherein said cumulative intensity measurement corresponds to the presence of a unique combination of presence or absence of said plurality of analytes in said sample; and
(e) determining whether said at least one of said plurality of analytes corresponding with said respiratory viral pathogen is present, in any unique combination of presence or absence, based on said cumulative intensity measurement, wherein the method does not require any step of immobilization of said plurality of polynucleotide analytes or mass spectrometry, and
wherein each analyte is derived from a respiratory viral pathogen.