	US 11,866,760 B2
	Microorganisms for the production of insect pheromones and related compounds
Micah Sheppard, Santa Monica, CA (US); Thomas Heel, Los Angeles, CA (US); Peter Meinhold, Topanga, CA (US); Keith Wampler, Santa Monica, CA (US); Pedro Coelho, Santa Monica, CA (US); and Effendi Leonard, Santa Monica, CA (US)
Assigned to Provivi, Inc., Santa Monica, CA (US)
Filed by Provivi, Inc., Santa Monica, CA (US)
Filed on Aug. 20, 2021, as Appl. No. 17/408,213.
Application 17/408,213 is a continuation of application No. 16/614,144, granted, now 11,104,921, issued on Aug. 31, 2021, previously published as PCT/US2018/033151, filed on May 17, 2018.
Claims priority of provisional application 62/507,654, filed on May 17, 2017.
Prior Publication US 2023/0034938 A1, Feb. 2, 2023
This patent is subject to a terminal disclaimer.
Int. Cl. C12P 7/64 (2022.01); C12N 9/04 (2006.01); C12N 9/02 (2006.01); C12N 9/10 (2006.01); C12N 15/81 (2006.01)

CPC C12P 7/64 (2013.01) [C12N 9/0006 (2013.01); C12N 9/0008 (2013.01); C12N 9/0071 (2013.01); C12N 9/1029 (2013.01); C12N 15/815 (2013.01)]

22 Claims

1. A method of producing a C₆-C₂₄fatty alcohol, said method comprising:

a) providing a recombinant Yarrowia lipolytica microorganism comprising a heterologous nucleic acid molecule encoding and expressing a Euglena fatty alcohol-forming fatty acyl reductase that catalyzes conversion of C₆-C₂₄fatty acyl-CoA into a corresponding mono- or poly-unsaturated C₆-C₂₄fatty alcohol; and

b) cultivating the recombinant Yarrowia lipolytica of (a) in a culture medium containing a carbon source feedstock and C₆-C₂₄fatty acyl-CoA;

wherein the C₆-C₂₄fatty acyl-CoA is converted to a C₆-C₂₄fatty alcohol by catalytic activity of the fatty alcohol-forming fatty acyl reductase.