CPC C12P 7/26 (2013.01) [C12N 15/113 (2013.01); C12N 15/63 (2013.01)] | 43 Claims |
1. A method of producing tagatose, comprising:
contacting fructose with a modified polypeptide comprising one or more modifications as compared to an unmodified polypeptide, the unmodified polypeptide being SEQ ID NO: 6, wherein
the modified polypeptide converts the fructose directly to the tagatose through epimerization at the carbon-4 position of the fructose,
the modified polypeptide comprises at least 90% identity to SEQ ID NO: 6, and
the modified polypeptide comprises H at position 85, D at position 121, H at position 247, D at position 250, and G at position 271.
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