US 11,866,758 B2
Methods and compositions for preparing tagatose from fructose
Alexandre Zanghellini, Seattle, WA (US); Kyle Roberts, Seattle, WA (US); Michael Charles Milner Cockrem, Madison, WI (US); and Christopher Dunckley, Seattle, WA (US)
Assigned to ARZEDA CORP., Seattle, WA (US)
Filed by Arzeda Corp., Seattle, WA (US)
Filed on Jun. 8, 2021, as Appl. No. 17/341,813.
Application 17/341,813 is a continuation of application No. 16/503,092, filed on Jul. 3, 2019, granted, now 11,060,119, issued on Jul. 13, 2021.
Claims priority of provisional application 62/693,681, filed on Jul. 3, 2018.
Claims priority of provisional application 62/693,660, filed on Jul. 3, 2018.
Prior Publication US 2022/0025413 A1, Jan. 27, 2022
This patent is subject to a terminal disclaimer.
Int. Cl. C12P 7/26 (2006.01); C12N 15/113 (2010.01); C12N 15/63 (2006.01)
CPC C12P 7/26 (2013.01) [C12N 15/113 (2013.01); C12N 15/63 (2013.01)] 43 Claims
 
1. A method of producing tagatose, comprising:
contacting fructose with a modified polypeptide comprising one or more modifications as compared to an unmodified polypeptide, the unmodified polypeptide being SEQ ID NO: 6, wherein
the modified polypeptide converts the fructose directly to the tagatose through epimerization at the carbon-4 position of the fructose,
the modified polypeptide comprises at least 90% identity to SEQ ID NO: 6, and
the modified polypeptide comprises H at position 85, D at position 121, H at position 247, D at position 250, and G at position 271.