	US 11,866,757 B2
	Plasma-driven biocatalysis
Julia Bandow, Witten (DE); Abdulkadir Yayci, Bochum (DE); Marco Krewing, Hamburg (DE); Robert Kourist, Graz (AT); and Alvaro Gomez Baraibar, Bochum (DE)
Assigned to Ruhr-Universitaet Bochum, Bochum (DE)
Appl. No. 17/254,929
Filed by Ruhr-Universitaet Bochum, Bochum (DE)
PCT Filed Jun. 11, 2019, PCT No. PCT/EP2019/065220 § 371(c)(1), (2) Date Dec. 22, 2020, PCT Pub. No. WO2020/007576, PCT Pub. Date Jan. 9, 2020.
Claims priority of application No. 10 2018 116 052.6 (DE), filed on Jul. 3, 2018.
Prior Publication US 2021/0115479 A1, Apr. 22, 2021
Int. Cl. C12P 7/22 (2006.01); C12N 9/04 (2006.01); C12N 9/08 (2006.01); C12N 9/02 (2006.01)

CPC C12P 7/22 (2013.01) [C12N 9/0006 (2013.01); C12N 9/0065 (2013.01); C12N 9/0069 (2013.01)]

11 Claims

1. A method for enzymatically oxidizing or hydroxylating an organic compound, wherein the method comprises:

treating an aqueous liquid with a plasma device to obtain an aqueous liquid comprising H₂O₂to form an enzymatically oxidized or hydroxylated organic compound; wherein the aqueous liquid comprises at least one enzyme, at least one organic compound, and optionally at least one solvent; wherein the at least one solvent is not water; wherein the at least one enzyme is immobilized on a solid support and positioned at a distance from the surface of the aqueous liquid in proximity to the plasma device where the distance ranges from 1 mm to 20 cm; and

optionally extracting the aqueous liquid to isolate the oxidized or hydroxylated organic compound.