US 11,866,756 B2
Methods for co-producing erythritol and arabinose by using xylose mother liquor
Aijuan Wu, Quzhou (CN); Jiaxing Luo, Quzhou (CN); Changhui Hu, Quzhou (CN); Mingqian Yang, Quzhou (CN); Chengjun Liao, Quzhou (CN); Yi Zheng, Quzhou (CN); Shuncheng Fang, Quzhou (CN); and Mian Li, Quzhou (CN)
Assigned to ZHEJIANG HUAKANG PHARMACEUTICAL CO., LTD., Quzhou (CN)
Filed by ZHEJIANG HUAKANG PHARMACEUTICAL CO., LTD., Zhejiang (CN)
Filed on Jul. 18, 2023, as Appl. No. 18/354,044.
Application 18/354,044 is a continuation of application No. PCT/CN2022/133112, filed on Nov. 21, 2022.
Claims priority of application No. 202111606216.9 (CN), filed on Dec. 26, 2021.
Prior Publication US 2023/0357805 A1, Nov. 9, 2023
Int. Cl. C13K 13/00 (2006.01); C12N 1/16 (2006.01); C07C 31/12 (2006.01); C12P 7/04 (2006.01); C12P 7/02 (2006.01); C10L 1/02 (2006.01); C12P 19/02 (2006.01); C12P 7/18 (2006.01); C12R 1/85 (2006.01)
CPC C12P 7/18 (2013.01) [C12N 1/165 (2021.05); C12P 19/02 (2013.01); C12R 2001/85 (2021.05)] 6 Claims
 
1. A method for co-producing erythritol and arabinose by utilizing a xylose mother liquor, comprising:
obtaining a xylose extract having a high content of xylose component and a xylose raffinate having a high content of glucose component respectively by separating the xylose mother liquor through a first simulated moving bed chromatography, and obtaining xylose crystals by concentrating and crystallizing the xylose extract;
obtaining a concentrated xylose raffinate by concentrating the xylose raffinate, and obtaining a glucose mixture by blending the concentrated xylose raffinate with liquid glucose or crystallized glucose, wherein the concentrated xylose raffinate has a solid content of 30% W/V-50% W/V and a glucose content of 9% W/V-14% W/V, the glucose mixture has a glucose content of 40% W/V-50% W/V;
obtaining a fermentation broth by inoculating a pre-prepared seed solution of a Yarrowia lipolytica into a fermentation medium of a fermenter, while adding the glucose mixture for fermenting, wherein a glucose content of the fermentation broth is less than 0.3% W/V;
obtaining a fermentation filtrate by filtering the fermentation broth, and obtaining erythritol crystals and a centrifuged erythritol mother liquor respectively by decolorizing, ion changing, concentrating, centrifuging and crystallizing a part of the fermentation filtrate in sequence;
obtaining an erythritol extract having a high content of an erythritol component and an erythritol raffinate having a high content of an arabinose component respectively by separating the centrifuged erythritol mother liquor through a second simulated moving bed chromatography, and mixing the erythritol extract with the other part of the fermentation filtrate; and
obtaining arabinose crystals by decolorizing, ion changing, concentrating and crystallizing the erythritol raffinate in sequence.