	US 11,866,751 B2
	Yeast expressing a heterologous alpha-amylase for ethanol production
Michael Glenn Catlett, West Sacramento, CA (US); Monica Tassone, West Sacramento, CA (US); Paul Vincent Harris, Carnation, WA (US); Robert Lyle Osborne, Raleigh, NC (US); Ryoko Kataoka, Longmont, CO (US); Shiro Fukuyama, Chiba (JP); and Tomoko Matsui, Chiba (JP)
Assigned to Novozymes A/S, Bagsvaerd (DK)
Appl. No. 17/260,516
Filed by Novozymes A/S, Bagsvaerd (DK)
PCT Filed Jul. 22, 2019, PCT No. PCT/US2019/042870 § 371(c)(1), (2) Date Jan. 14, 2021, PCT Pub. No. WO2020/023411, PCT Pub. Date Jan. 30, 2020.
Claims priority of provisional application 62/703,103, filed on Jul. 25, 2018.
Prior Publication US 2021/0261988 A1, Aug. 26, 2021
Int. Cl. C12P 7/06 (2006.01)

CPC C12P 7/06 (2013.01) [C12P 2203/00 (2013.01); C12Y 302/01001 (2013.01); C12Y 302/01093 (2013.01)]

20 Claims

1. A method of producing a fermentation product from a starch-containing or cellulosic-containing material comprising:

(a) saccharifying the starch-containing or cellulosic-containing material; and

(b) fermenting the saccharified material of step (a) with a recombinant yeast cell;

wherein the recombinant yeast cell comprises a heterologous polynucleotide encoding an alpha-amylase having a mature polypeptide sequence with at least 95% sequence identity to the amino acid sequence of SEQ ID NO: 126.