US 11,866,738 B2
UDP-dependent glycosyltransferase for high efficiency production of rebaudiosides
Lishan Zhao, Emeryville, CA (US); Wenzong Li, Emeryville, CA (US); Gale Wichmann, Berkeley, CA (US); Aditi Khankhoje, Emeryville, CA (US); Chantal Garcia De Gonzalo, Richmond, CA (US); Tina Mahatdejkul-Meadows, Milpitas, CA (US); Shaina Jackson, Oakland, CA (US); Michael Leavell, Richmond, CA (US); and Darren Platt, San Francisco, CA (US)
Assigned to AMYRIS, INC., Emeryville, CA (US)
Filed by AMYRIS, INC., Emeryville, CA (US)
Filed on Jul. 22, 2021, as Appl. No. 17/382,963.
Application 17/382,963 is a division of application No. 16/323,756, granted, now 11,091,743, previously published as PCT/US2017/046637, filed on Aug. 11, 2017.
Claims priority of provisional application 62/374,408, filed on Aug. 12, 2016.
Prior Publication US 2021/0355458 A1, Nov. 18, 2021
This patent is subject to a terminal disclaimer.
Int. Cl. C12N 9/10 (2006.01); A23L 2/60 (2006.01); C12P 19/56 (2006.01); C12N 15/52 (2006.01); A23L 27/30 (2016.01); C12N 15/81 (2006.01); C12P 19/18 (2006.01)
CPC C12N 9/1051 (2013.01) [A23L 2/60 (2013.01); A23L 27/36 (2016.08); C12N 15/52 (2013.01); C12N 15/81 (2013.01); C12P 19/18 (2013.01); C12P 19/56 (2013.01); C12Y 204/01017 (2013.01); A23V 2002/00 (2013.01)] 25 Claims
 
1. A genetically modified yeast host cell comprising a heterologous nucleic acid encoding a UDP-glycosyltransferase comprising an amino acid sequence having at least 80% sequence identity to SEQ ID NO: 11, wherein the genetically modified host cell is capable of converting Rebaudioside A (RebA) to Rebaudioside D (RebD) at an efficiency of greater than 90%.