	US 11,866,737 B2
	2-isopropylmalate synthetase and engineering bacteria and application thereof
Chenglin Zhang, Tianjin (CN); Qingyang Xu, Tianjin (CN); Yanjun Li, Tianjin (CN); Yu Zhang, Tianjin (CN); Yingzi Li, Tianjin (CN); Fuzhou Zhu, Tianjin (CN); Nan Lu, Tianjin (CN); Shibao Han, Tianjin (CN); Xierong Dong, Tianjin (CN); Zishen Wang, Tianjin (CN); Hao Xu, Tianjin (CN); and Ziyi Li, Tianjin (CN)
Assigned to Tianjin University of Science and Technology, Tianjin (CN)
Filed by Tianjin University of Science and Technology, Tianjin (CN)
Filed on Dec. 29, 2020, as Appl. No. 17/137,327.
Application 17/137,327 is a continuation of application No. PCT/CN2020/112038, filed on Aug. 28, 2020.
Prior Publication US 2021/0189354 A1, Jun. 24, 2021
Int. Cl. C12N 9/10 (2006.01); C12P 13/06 (2006.01)

CPC C12N 9/1025 (2013.01) [C12P 13/06 (2013.01); C12Y 203/03013 (2013.01)]

11 Claims

1. A genetically engineered bacterium for producing L-leucine, the genetically engineered bacterium is obtained by overexpressing a gene leuA^Mencoding 2-isopropyl malate synthase, a gene ilvBN^Mencoding acetohydroxy acid synthase for relieving feedback inhibition by L-isoleucine, a gene leuB encoding 3-isopropyl malate dehydrogenase and a gene leuCD encoding 3-isopropyl malate dehydratase in a host cell, wherein the nucleotide sequence of the gene leuA^Mis shown as SEQ ID NO. 2.