CPC C12N 9/0036 (2013.01) [C07K 14/195 (2013.01); C07K 14/335 (2013.01); C12N 5/0602 (2013.01); C12N 5/10 (2013.01); C12N 15/52 (2013.01); C12N 15/85 (2013.01); C12Y 106/03001 (2013.01); A61K 9/127 (2013.01); A61K 38/162 (2013.01); A61K 38/1709 (2013.01); A61K 38/1774 (2013.01); A61K 47/18 (2013.01); A61K 47/42 (2013.01); A61K 47/66 (2017.08); A61P 25/16 (2018.01); C07K 2319/07 (2013.01); C07K 2319/43 (2013.01); C12N 15/113 (2013.01); C12N 15/62 (2013.01)] | 5 Claims |
1. A mammalian cell comprising a nucleic acid encoding a water-forming NADH oxidase bound to a targeting peptide that localizes the water-forming NADH oxidase to mitochondria, wherein the water-forming NADH oxidase comprises an NADH-binding site comprising (i) a Rossmann fold domain having a dinucleotide-binding motif defined by the peptide G-x-G-x-x-G/A, wherein each x represents any amino acid, and (ii) a cofactor specificity loop, wherein the water-forming NADH oxidase is a bacterial water-forming NADH oxidase.
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