	US 11,866,734 B2
	Super enhancer for driving pluripotency network and stemness circuitry
Shih-Hwa Chiou, Taipei (TW); Ping-Hsing Tsai, Taipei (TW); and Yueh Chien, Taipei (TW)
Assigned to TAIPEI VETERANS GENERAL HOSPITAL, Taipei (TW)
Filed by Taipei Veterans General Hospital, Taipei (TW)
Filed on Aug. 26, 2020, as Appl. No. 17/003,799.
Claims priority of provisional application 62/891,538, filed on Aug. 26, 2019.
Prior Publication US 2021/0102174 A1, Apr. 8, 2021
Int. Cl. C12N 5/074 (2010.01); C12N 15/86 (2006.01)

CPC C12N 5/0696 (2013.01) [C12N 15/86 (2013.01); C12N 2501/602 (2013.01); C12N 2501/603 (2013.01); C12N 2501/605 (2013.01)]

12 Claims

1. A method for preparing induced pluripotent stem cells (iPSCs) from somatic cells, comprising:

(a) transfecting or transducing into isolated somatic cells (i) Ash21-a and (ii) Klf4, Sox2, and Oct4; and

(b) culturing the isolated somatic cells as obtained in step (a) under appropriate conditions, thereby converting the somatic cells into iPSCs and maintaining pluripotency and self-renewal ability.