US 11,866,732 B2
Separation, dissociation and/or disaggregation of cells using shockwaves or mechanical impacts
John Chi, Alhambra, CA (US); and Ben-Chen Chi, Fullerton, CA (US)
Assigned to Synova Life Sciences, Inc., Pasadena, CA (US)
Appl. No. 16/085,200
Filed by Synova Life Sciences, Inc., Pasadena, CA (US)
PCT Filed Mar. 17, 2017, PCT No. PCT/US2017/023084
§ 371(c)(1), (2) Date Sep. 14, 2018,
PCT Pub. No. WO2017/161343, PCT Pub. Date Sep. 21, 2017.
Claims priority of provisional application 62/309,774, filed on Mar. 17, 2016.
Prior Publication US 2019/0071644 A1, Mar. 7, 2019
Int. Cl. C12M 1/33 (2006.01); C12N 5/0775 (2010.01)
CPC C12N 5/0667 (2013.01) [C12M 45/02 (2013.01); C12N 2509/10 (2013.01)] 14 Claims
OG exemplary drawing
 
1. A tissue processing device, comprising:
a processing container for containing raw adipose tissue;
a platform onto which the processing container is attached;
an impact arm; and
a variable speed motor that drives the impact arm such t hat the impact arm articulates up and down at a rate of up to 30,000 rpm, making physical contact with the processing container;
wherein;
the articulation of the impact arm generates mechanical impacts that are imparted to an outside surface of the processing container that break down the raw adipose tissue and separate stem cells from the adipose tissue; and
the motor controls the speed at which the impact arm makes contact with the processing container; and
wherein the motor comprises gearing capable of reducing the speed of the impact arm.