US 11,866,719 B1
Heterologous integration of regulatory elements to alter gene expression in wheat cells and wheat plants
Tomás Cermák, Brookline, MA (US); Richard Bailey Flavell, Malibu, CA (US); Michael Andreas Kock, Rheinfelden (DE); Eva Konecna, Brookline, MA (US); Yajie Niu, Lexington, MA (US); Michael Lee Nuccio, Salem, NH (US); Jennifer Raji, Waltham, MA (US); Randall William Shultz, Acton, MA (US); Davide Sosso, Cambridge, MA (US); Maria Margarita D. Unson, Pawcatuck, CT (US); John P. Casey, Jr., Boston, MA (US); and Jonathan Dallas George Jones, Norwich (GB)
Assigned to INARI AGRICULTURE TECHNOLOGY, INC., Cambridge, MA (US)
Filed by INARI AGRICULTURE TECHNOLOGY, INC., Cambridge, MA (US)
Filed on Jun. 4, 2019, as Appl. No. 16/430,958.
Claims priority of provisional application 62/680,447, filed on Jun. 4, 2018.
Int. Cl. C12N 15/82 (2006.01)
CPC C12N 15/8241 (2013.01) [C12N 15/827 (2013.01); C12N 15/8213 (2013.01); C12N 15/8216 (2013.01); C12N 15/8266 (2013.01); C12N 15/8269 (2013.01); C12N 15/8271 (2013.01); C12N 15/8279 (2013.01)] 14 Claims
 
1. A wheat plant, or a cell thereof, having a modified genome that results in the wheat plant exhibiting modified architecture in comparison to an unmodified wheat plant having a reference genome, wherein the modified genome differs from the reference genome by comprising at least one predetermined modification of a gene, wherein the at least one predetermined modification comprises heterologous integration of a nucleic acid sequence that is encoded by at least one donor polynucleotide molecule and that comprises a regulatory element, at a predetermined locus between 1-1000 nucleotides upstream of the start codon of the coding sequence of the gene in the reference genome, wherein the regulatory element comprises SEQ ID NO: 231, and wherein the predetermined modification of the gene results in increased expression of the gene relative to expression of the gene in the reference genome.