US 11,866,715 B2
Pichia pastoris strains for producing predominantly homogeneous glycan structure
Kurt R. Gehlsen, Tucson, AZ (US); and Thomas G. Chappell, San Marcos, CA (US)
Assigned to RESEARCH CORPORATION TECHNOLOGIES, INC., Tucson, AZ (US)
Filed by Research Corporation Technologies, Inc., Tucson, AZ (US)
Filed on Nov. 17, 2021, as Appl. No. 17/528,619.
Application 17/528,619 is a continuation of application No. 16/801,466, filed on Feb. 26, 2020, granted, now 11,220,692.
Application 16/801,466 is a continuation of application No. 16/404,838, filed on May 7, 2019, granted, now 10,612,033, issued on Apr. 7, 2020.
Application 16/404,838 is a continuation of application No. 15/444,870, filed on Feb. 28, 2017, granted, now 10,329,572, issued on Jun. 25, 2019.
Application 15/444,870 is a continuation of application No. 14/437,683, granted, now 9,617,550, issued on Apr. 11, 2017, previously published as PCT/US2013/066335, filed on Oct. 23, 2013.
Claims priority of provisional application 61/717,423, filed on Oct. 23, 2012.
Prior Publication US 2022/0145311 A1, May 12, 2022
This patent is subject to a terminal disclaimer.
Int. Cl. C12N 15/81 (2006.01); C12N 9/10 (2006.01); C12N 9/24 (2006.01)
CPC C12N 15/815 (2013.01) [C12N 9/1051 (2013.01); C12N 9/2488 (2013.01); C12Y 204/01232 (2013.01); C12Y 302/0113 (2013.01)] 14 Claims
 
1. An engineered strain of Pichia pastoris, comprising:
a mutant OCH1 allele comprising a nucleotide sequence coding for a mutant OCH1 protein,
wherein said mutant OCH1 protein comprises a catalytic domain comprising an amino acid sequence that is at least 95% identical to the catalytic domain of a wild type OCH1 protein as set forth in amino acids 54-404 of SEQ ID NO: 2, wherein said mutant OCH1 protein lacks an N-terminal sequence for targeting the mutant OCH1 protein to the Golgi apparatus and has α-1,6-mannosyl transferase activity.