CPC C12N 15/815 (2013.01) [C12N 9/1051 (2013.01); C12N 9/2488 (2013.01); C12Y 204/01232 (2013.01); C12Y 302/0113 (2013.01)] | 14 Claims |
1. An engineered strain of Pichia pastoris, comprising:
a mutant OCH1 allele comprising a nucleotide sequence coding for a mutant OCH1 protein,
wherein said mutant OCH1 protein comprises a catalytic domain comprising an amino acid sequence that is at least 95% identical to the catalytic domain of a wild type OCH1 protein as set forth in amino acids 54-404 of SEQ ID NO: 2, wherein said mutant OCH1 protein lacks an N-terminal sequence for targeting the mutant OCH1 protein to the Golgi apparatus and has α-1,6-mannosyl transferase activity.
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