US 11,866,703 B2
Method for knocking out N-myristoyltransferase (NMT) gene from Eimeria tenella
Jianping Cai, Lanzhou (CN); Zigang Qu, Lanzhou (CN); Xiao Xu, Lanzhou (CN); Jing Wang, Lanzhou (CN); Zhenxing Gong, Lanzhou (CN); Heng Wang, Lanzhou (CN); Xueyang He, Lanzhou (CN); and Wenqing Wang, Lanzhou (CN)
Assigned to LANZHOU VETERINARY RESEARCH INSTITUTE, CHINESE ACADEMY OF AGRICULTURAL SCIENCES, Lanzhou (CN)
Filed by LANZHOU VETERINARY RESEARCH INST., CHINESE ACADEMY OF AGRICULTURAL SCIENCES, Lanzhou (CN)
Filed on Dec. 23, 2020, as Appl. No. 17/133,300.
Claims priority of application No. 201911365073.X (CN), filed on Dec. 26, 2019.
Prior Publication US 2021/0198670 A1, Jul. 1, 2021
Int. Cl. C12N 15/113 (2010.01); C12N 9/22 (2006.01); C12N 15/79 (2006.01); C12N 15/10 (2006.01)
CPC C12N 15/113 (2013.01) [C12N 9/22 (2013.01); C12N 15/1031 (2013.01); C12N 15/79 (2013.01); C12N 2310/20 (2017.05)] 10 Claims
 
1. A method for knocking out an N-myristoyltransferase (NMT) gene from Eimeria tenella, comprising:
a. mixing sporozoites of Eimeria tenella with a pCRISPR::EtNMT plasmid and a pEtNMT::DHFR plasmid to obtain a sporozoites-plasmids mixture, and
b. subjecting the sporozoites-plasmids mixture to electrotransformation to obtain NMT gene-knockout Eimeria tenella.