CPC C12N 15/113 (2013.01) [C12N 9/22 (2013.01); C12N 15/1031 (2013.01); C12N 15/79 (2013.01); C12N 2310/20 (2017.05)] | 10 Claims |
1. A method for knocking out an N-myristoyltransferase (NMT) gene from Eimeria tenella, comprising:
a. mixing sporozoites of Eimeria tenella with a pCRISPR::EtNMT plasmid and a pEtNMT::DHFR plasmid to obtain a sporozoites-plasmids mixture, and
b. subjecting the sporozoites-plasmids mixture to electrotransformation to obtain NMT gene-knockout Eimeria tenella.
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