US 11,866,692 B2
Fermentation fungal substance of astragalus membranaceus paecilomyces cicadae and its use
Jiayu Zhang, Yantai (CN); Long Dai, Yantai (CN); Shaoping Wang, Yantai (CN); Zikai Geng, Yantai (CN); and Yuqi Wang, Yantai (CN)
Assigned to BinZhou Medical University
Appl. No. 17/776,529
Filed by BinZhou Medical University, Yantai (CN)
PCT Filed Sep. 2, 2020, PCT No. PCT/CN2020/112945
§ 371(c)(1), (2) Date May 12, 2022,
PCT Pub. No. WO2021/093425, PCT Pub. Date May 20, 2021.
Claims priority of application No. 201911119896.4 (CN), filed on Nov. 15, 2019.
Prior Publication US 2022/0380719 A1, Dec. 1, 2022
Int. Cl. C12N 1/14 (2006.01)
CPC C12N 1/14 (2013.01) [C12N 2523/00 (2013.01)] 7 Claims
 
1. A method for preparing a fungal fermentation substance of Astragalus membranaceus/Paecilomyces cicadae comprising:
(1) mixing an Astragalus membranaceus powder with water, and then subjecting to a sterilization operation to obtain a solid medium of Astragalus membranaceus; wherein a weight ratio of the Astragalus membranaceus powder to water is 1.5-3:1;
(2) inoculating an Paecilomyces cicadae into the solid medium of Astragalus membranaceus, and culturing at a culture temperature of 26-28° C. and a relative humidity of 70-90% for 20-24 days, so as to obtain the fungal fermentation substance of Astragalus membranaceus/Paecilomyces cicadae;
wherein the Paecilomyces cicadae has an inoculation amount of 5-20 wt %, based on the weight of the Astragalus membranaceus powder;
(3) obtaining the fungal fermentation substance of Astragalus membranaceus/Paecilomyces cicadae by solid fermentation with the Astragalus membranaceus powder as a fermentation substrate and the Paecilomyces cicadae as a fermentation strain;
wherein the Astragalus membranaceus powder has a particle size of 8-14 mesh for conducive growth of Paecilomyces cicadae and in step (1) the weight ratio of the Astragalus membranaceus powder to water is for inoculated Paecilomyces cicadae to fully contact with the Astragalus membranaceus powder resulting in a better fermentation effect;
wherein a polysaccharide content in the fungal fermentation substance of the Astragalus membranaceus/Paecilomyces cicadae is significantly increased as compared to Astragalus membranaceus.