US 11,866,687 B2
Robotic platform for high throughput injections into intact tissue
Suhasa Kodandaramaiah, Minneapolis, MN (US); Elena Taverna, Dresden (DE); Gabriella Shull, Minneapolis, MN (US); and Wieland Huttner, Dresden (DE)
Assigned to Regents of the University of Minnesota, Minneapolis, MN (US)
Appl. No. 16/642,986
Filed by Regents of the University of Minnesota, Minneapolis, MN (US); and Max-Planck-Gesellschaft zur Foerderung der Wissenschaften e.V., Munich (DE)
PCT Filed Sep. 6, 2018, PCT No. PCT/US2018/049728
§ 371(c)(1), (2) Date Feb. 28, 2020,
PCT Pub. No. WO2019/051072, PCT Pub. Date Mar. 14, 2019.
Claims priority of provisional application 62/554,993, filed on Sep. 6, 2017.
Prior Publication US 2020/0308531 A1, Oct. 1, 2020
Int. Cl. G01N 35/00 (2006.01); C12M 1/36 (2006.01); C12M 3/00 (2006.01); C12M 3/06 (2006.01); C12M 1/00 (2006.01); C12M 1/26 (2006.01); C12M 1/34 (2006.01); G01N 35/10 (2006.01); B01L 3/02 (2006.01); B01L 9/00 (2006.01); C12N 15/87 (2006.01); C12N 15/89 (2006.01)
CPC C12M 41/48 (2013.01) [B01L 3/0237 (2013.01); B01L 9/54 (2013.01); C12M 21/08 (2013.01); C12M 23/16 (2013.01); C12M 23/50 (2013.01); C12M 33/04 (2013.01); C12M 41/08 (2013.01); C12M 41/40 (2013.01); G01N 35/00693 (2013.01); G01N 35/109 (2013.01); C12N 15/87 (2013.01); C12N 15/89 (2013.01); G01N 35/1002 (2013.01); G01N 2035/1039 (2013.01)] 18 Claims
OG exemplary drawing
 
1. A system for injecting a substance into cells of a tissue sample, the system comprising:
a robotic manipulator apparatus configured to hold and position a micropipette;
a pressure controller;
a microscope camera; and
a computing device configured to:
receive image data from the microscope camera, wherein the image data comprises an image of the tissue sample;
output a user interface for display, wherein the user interface contains the image of the tissue sample;
receive, via the user interface, an indication of a trajectory line drawn by a user on the image of the tissue sample;
control the robotic manipulator apparatus to move a tip of the micropipette along a path defined by the trajectory line; and
control the pressure controller to inject a gas into the micropipette to eject the substance out of the micropipette at one or more points along the path defined by the trajectory line.