US 11,866,469 B2
DNA binding proteins and uses thereof
Ci-Di Chen, Boston, MA (US)
Assigned to Klogenix LLC, Boston, MA (US)
Appl. No. 17/287,218
Filed by Klogenix LLC, Boston, MA (US)
PCT Filed Dec. 16, 2019, PCT No. PCT/US2019/066535
§ 371(c)(1), (2) Date Apr. 21, 2021,
PCT Pub. No. WO2020/163017, PCT Pub. Date Aug. 13, 2020.
Claims priority of provisional application 62/860,407, filed on Jun. 12, 2019.
Claims priority of provisional application 62/801,889, filed on Feb. 6, 2019.
Prior Publication US 2021/0380648 A1, Dec. 9, 2021
Int. Cl. C07K 14/47 (2006.01); A61K 48/00 (2006.01); A61K 38/00 (2006.01)
CPC C07K 14/47 (2013.01) [A61K 48/005 (2013.01); A61K 38/00 (2013.01); C07K 2319/81 (2013.01)] 6 Claims
 
1. A method of increasing expression of a Klotho gene in a cell the method comprising administering to the cell a DNA binding protein comprising or attached to a transcriptional activation domain wherein the DNA binding protein binds to a target sequence within or near the Klotho gene and thereby increases expression of the Klotho gene, wherein the DNA binding protein is a zinc finger protein comprising:
a ZF1 zinc finger comprising the sequence set forth in SEQ ID NO 8, a ZF2 zinc finger comprising the sequence set forth in SEQ ID NO 16, a ZF3 zinc finger comprising the sequence set forth in SEQ ID NO 18, a ZF4 zinc finger comprising the sequence set forth in SEQ ID NO. 16, a ZF5 zinc finger comprising the sequence set forth in SEQ ID NO 3, a ZF6 zinc finger comprising the sequence set forth in SEQ ID NO 8 and a ZF7 zinc finger comprising the sequence set forth in SEQ ID NO 3.