US 11,866,464 B2
Site-specific bio-conjugation methods and compositions useful for nanopore systems
Dmitriy Gremyachinskiy, San Francisco, CA (US); and Christos Tzitzilonis, Redwood City, CA (US)
Assigned to Roche Sequencing Solutions, Inc., Pleasanton, CA (US)
Filed by Roche Sequencing Solutions, Inc., Pleasanton, CA (US)
Filed on Apr. 9, 2021, as Appl. No. 17/226,378.
Application 17/226,378 is a division of application No. 16/134,179, filed on Sep. 18, 2018, granted, now 11,001,611.
Application 16/134,179 is a continuation of application No. PCT/EP2017/057002, filed on Mar. 23, 2017.
Claims priority of provisional application 62/313,086, filed on Mar. 24, 2016.
Prior Publication US 2021/0230232 A1, Jul. 29, 2021
Int. Cl. C07K 14/315 (2006.01); C07K 1/107 (2006.01); A61K 47/64 (2017.01); C07K 19/00 (2006.01); C12N 9/12 (2006.01); C12N 9/52 (2006.01); C12N 15/62 (2006.01); C12Q 1/68 (2018.01)
CPC C07K 14/315 (2013.01) [A61K 47/64 (2017.08); A61K 47/6415 (2017.08); C07K 1/1072 (2013.01); C07K 1/1077 (2013.01); C07K 19/00 (2013.01); C12N 9/1205 (2013.01); C12N 9/1241 (2013.01); C12N 9/52 (2013.01); C12N 15/62 (2013.01); C12Q 1/68 (2013.01); C07K 2319/03 (2013.01); C07K 2319/20 (2013.01); C12Y 207/07 (2013.01)] 9 Claims
 
1. A composition comprising a modified pore-forming protein of structural formula (IVa)

OG Complex Work Unit Chemistry
wherein,
S is a sulfur atom of a thiol group of the pore forming protein;
A is a thiol reactive group;
LA is a linker; and
X is a click chemistry reactive group;
Y is a click chemistry reactive group that undergoes a click chemistry reaction with the reactive group X;
LB is a linker; and
B is a reactive group, wherein the reactive group B comprises a SpyTag peptide or a SpyCatcher protein.