US 11,865,541 B2
Dual-depth thermoplastic microfluidic device and related systems and methods
Rolf Muller, Del Mar, CA (US); and Mateusz Hupert, Lawrence, KS (US)
Assigned to BioFluidica, Inc., San Diego, CA (US)
Filed by BioFluidica, Inc., San Diego, CA (US)
Filed on Dec. 12, 2022, as Appl. No. 18/079,860.
Application 18/079,860 is a continuation of application No. PCT/US2021/037297, filed on Jun. 14, 2021.
Claims priority of provisional application 63/038,492, filed on Jun. 12, 2020.
Prior Publication US 2023/0211342 A1, Jul. 6, 2023
Int. Cl. B01L 3/00 (2006.01); B01L 3/02 (2006.01)
CPC B01L 3/502753 (2013.01) [B01L 3/021 (2013.01); B01L 3/502715 (2013.01); B01L 2200/027 (2013.01); B01L 2200/0647 (2013.01); B01L 2200/12 (2013.01); B01L 2300/0816 (2013.01); B01L 2300/12 (2013.01); B01L 2400/0487 (2013.01); B01L 2400/086 (2013.01)] 30 Claims
OG exemplary drawing
 
1. A dual-depth thermoplastic microfluidic device comprising:
a thermoplastic substrate comprising an inlet channel, an outlet channel, bifurcated channels, and one or more isolation beds comprising a plurality of microposts, wherein the one or more isolation beds are connected to the inlet channel and outlet channel by the bifurcated channels;
wherein each of the microposts has a height in the range of about 40 μm to about 60 μm, and a width in the range of about 5 μm to about 15 μm; and wherein at least a portion of the microposts are spaced apart by about 5 μm to about 15 μm;
wherein a cross-section of the bifurcated channels has a height in the range of about 40 μm to about 60 μm;
wherein a cross-section of the inlet channel has a height in the range of about 40 μm to about 500 μm, and a width in the range of 200 μm to about 500 μm; and
wherein a cross-section of the outlet channel has a height in the range of about 40 μm to about 500 μm, and a width in the range of 200 μm to about 500 μm;
wherein an aspect ratio of each of the inlet channel and outlet channel is about 1:4 to about 4:1; and
wherein the inlet channel, the outlet channel, the bifurcated channels, and the one or more isolation beds are a single dual-depth fluidic layer.