CPC B01L 3/502753 (2013.01) [B01L 3/021 (2013.01); B01L 3/502715 (2013.01); B01L 2200/027 (2013.01); B01L 2200/0647 (2013.01); B01L 2200/12 (2013.01); B01L 2300/0816 (2013.01); B01L 2300/12 (2013.01); B01L 2400/0487 (2013.01); B01L 2400/086 (2013.01)] | 30 Claims |
1. A dual-depth thermoplastic microfluidic device comprising:
a thermoplastic substrate comprising an inlet channel, an outlet channel, bifurcated channels, and one or more isolation beds comprising a plurality of microposts, wherein the one or more isolation beds are connected to the inlet channel and outlet channel by the bifurcated channels;
wherein each of the microposts has a height in the range of about 40 μm to about 60 μm, and a width in the range of about 5 μm to about 15 μm; and wherein at least a portion of the microposts are spaced apart by about 5 μm to about 15 μm;
wherein a cross-section of the bifurcated channels has a height in the range of about 40 μm to about 60 μm;
wherein a cross-section of the inlet channel has a height in the range of about 40 μm to about 500 μm, and a width in the range of 200 μm to about 500 μm; and
wherein a cross-section of the outlet channel has a height in the range of about 40 μm to about 500 μm, and a width in the range of 200 μm to about 500 μm;
wherein an aspect ratio of each of the inlet channel and outlet channel is about 1:4 to about 4:1; and
wherein the inlet channel, the outlet channel, the bifurcated channels, and the one or more isolation beds are a single dual-depth fluidic layer.
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