US 11,865,536 B2
Human lymphoid tissue-on-chip
Girija Goyal, Cambridge, MA (US); and Donald E. Ingber, Boston, MA (US)
Assigned to PRESIDENT AND FELLOWS OF HARVARD COLLEGE, Cambridge, MA (US)
Filed by President and Fellows of Harvard College, Cambridge, MA (US)
Filed on Apr. 27, 2022, as Appl. No. 17/730,615.
Application 17/730,615 is a continuation of application No. 16/318,591, granted, now 11,406,975, previously published as PCT/US2017/042657, filed on Jul. 18, 2017.
Claims priority of provisional application 62/398,646, filed on Sep. 23, 2016.
Claims priority of provisional application 62/363,579, filed on Jul. 18, 2016.
Prior Publication US 2022/0250055 A1, Aug. 11, 2022
Int. Cl. B01L 3/00 (2006.01); C12N 5/0781 (2010.01); C12N 5/0783 (2010.01); A61K 35/28 (2015.01); A61P 35/00 (2006.01); A61K 35/17 (2015.01); C12M 3/06 (2006.01); A61P 37/04 (2006.01); C12M 3/00 (2006.01); A61P 7/00 (2006.01); C12N 5/00 (2006.01); C12N 5/0786 (2010.01); C12N 5/078 (2010.01); C12N 5/071 (2010.01); C12N 5/09 (2010.01); G01N 33/50 (2006.01)
CPC B01L 3/5027 (2013.01) [A61K 35/17 (2013.01); A61K 35/28 (2013.01); A61P 7/00 (2018.01); A61P 35/00 (2018.01); A61P 37/04 (2018.01); C12M 21/08 (2013.01); C12M 23/16 (2013.01); C12N 5/0018 (2013.01); C12N 5/069 (2013.01); C12N 5/0635 (2013.01); C12N 5/0636 (2013.01); C12N 5/0645 (2013.01); C12N 5/0651 (2013.01); C12N 5/0693 (2013.01); G01N 33/5011 (2013.01); B01L 2300/0861 (2013.01); C12N 2531/00 (2013.01); C12N 2533/52 (2013.01); C12N 2533/54 (2013.01); C12N 2533/90 (2013.01)] 10 Claims
 
1. A microfluidic device comprising:
a body comprising a first microchannel, a first chamber and a second chamber, the first microchannel having an inlet and an outlet to an exterior of the body, the first chamber being in fluidic communication with the first microchannel and comprising a first matrix comprising B lymphocytes, and the second chamber comprising a second matrix comprising T lymphocytes.