US 11,865,196 B2
Engineered radioactive polymeric microsphere, and preparation and application thereof
Gang Liu, Xiamen (CN); Xiao Xu, Xiamen (CN); Hongjuan Ma, Shanghai (CN); Zhenwen Zhao, Xiamen (CN); Hu Chen, Xiamen (CN); Pan He, Xiamen (CN); Chao Liu, Xiamen (CN); Chengchao Chu, Xiamen (CN); and Dong Li, Xiamen (CN)
Assigned to Xiamen University, Xiamen (CN); and Shanghai University, Shanghai (CN)
Filed by Xiamen University, Xiamen (CN); and Shanghai University, Shanghai (CN)
Filed on Jun. 21, 2023, as Appl. No. 18/339,195.
Prior Publication US 2023/0346992 A1, Nov. 2, 2023
Int. Cl. A61P 35/00 (2006.01); A61K 51/12 (2006.01); C08F 257/02 (2006.01); C08K 3/06 (2006.01); A61N 5/10 (2006.01)
CPC A61K 51/1251 (2013.01) [A61N 5/1002 (2013.01); A61P 35/00 (2018.01); C08F 257/02 (2013.01); C08K 3/06 (2013.01); A61N 2005/1021 (2013.01)] 3 Claims
 
1. A method for preparing an engineered radioactive polymeric microsphere, comprising:
adding 10 g of a styrene monomer and 1 g of sodium dodecyl sulfate (SDS) to 20 mL of deionized water, followed by stirring at room temperature in a nitrogen atmosphere for 2 h to obtain a first reaction mixture;
heating the first reaction mixture to 50° C. and adding 2 g of dibenzoyl peroxide to the first reaction mixture, followed by reaction under stirring at a constant temperature for 6 h to obtain a second reaction mixture; and
subjecting the second reaction mixture to washing with ethanol and 60° C. water, and vacuum drying to obtain a crude polymeric microsphere;
weighing 3 g of the crude polymeric microsphere followed by irradiation in the exposure to a cobalt source at room temperature under an air atmosphere to obtain an irradiated polymeric microsphere, wherein a radiation absorbed dose is 100 kGy; adding 60 mL of deionized water and 10 mL of vinylphosphonic acid to a conical flask followed by stirring for 1 h to obtain a vinylphosphonic acid solution; adding 3 g of the irradiated polymeric microsphere to the vinylphosphonic acid solution followed by nitrogen feeding for 20 min to remove oxygen and sealing to obtain a third reaction mixture; subjecting the third reaction mixture to reaction in a 55° C. water bath for 3 h and vacuum filtration to obtain a grafted polymeric microsphere; and subjecting the grafted polymeric microsphere to washing with deionized water and drying in a vacuum oven for 24 h to obtain a phosphate-based polymeric microsphere; and
mixing 0.5 g of the phosphate-based polymeric microsphere with 1 mL of a 0.5 mCi/μL 177LuCl3 solution at room temperature under ultrasonic oscillation for 30 min to obtain an engineered phosphate-based 177Lu polymeric microsphere with a diameter of 40 μm.