CPC A61K 48/0091 (2013.01) [A61K 9/1272 (2013.01); A61K 9/1277 (2013.01); A61K 31/7105 (2013.01); A61K 38/465 (2013.01); A61K 47/10 (2013.01); A61K 47/18 (2013.01); A61K 47/186 (2013.01); A61K 47/24 (2013.01); A61K 47/28 (2013.01)] | 3 Claims |
1. A composition comprising at least one cargo entrapped in a lipid-based transfection competent vesicle (TCV), wherein:
(a) the lipid-based TCV consists of 1,2-Dioleyloxy-3-dimethylamino-propane (DODMA) at 20-30 mol %, 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE) at 20-30 mol %, 1,2-distearoyl-sn-glycero-3-phosphocholine (DSPC) at 10 mol %, and cholesterol at 40 mol %; and
(b) the at least one cargo comprises a Cas9 protein and a guide RNA.
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