CPC A61K 48/005 (2013.01) [A61K 9/0085 (2013.01); C07K 16/32 (2013.01); C07K 2317/24 (2013.01); C12N 2750/14143 (2013.01)] | 16 Claims |
1. A composition comprising at least one recombinant adeno-associated virus (rAAV) and at least one pharmaceutically acceptable carrier, preservative, and/or stabilizer, wherein the at least one rAAV comprises a vector genome in an AAV capsid,
wherein the vector genome is a nucleic acid molecule which comprises an AAV 5′ ITR, an expression cassette, and an AAV 3′ ITR,
wherein said expression cassette comprises:
(i) a coding sequence for an anti-Her2 immunoglobulin heavy chain having an IL-2 leader peptide, a heavy chain variable region having an amino acid sequence of SEQ ID NO: 18 and a heavy chain constant region,
(ii) a linker sequence,
(iii) a coding sequence for an anti-Her2 immunoglobulin light chain having an IL-2 leader peptide, a light chain variable region having an amino acid sequence of SEQ ID NO: 20, and a light chain constant region, and
(iv) regulatory sequences which direct expression of the heavy chain and the light chain, and
wherein following expression in the cell, a anti-Her2 heavy chain and the anti-Her2 light chain form an anti-Her2 antibody in vivo.
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