US 11,865,164 B2
Pharmaceutical composition for treating cancer, containing guide RNA and endonuclease as active ingredients
Sunghwa Choe, Seoul (KR); Mi Jin Park, Seoul (KR); Aiden Yeonghoon Park, Seoul (KR); Jung Hak Lim, Seoul (KR); Dong Wook Kim, Seoul (KR); Sungyong In, Seoul (KR); and Jongjin Park, West Lafayette, IN (US)
Assigned to G+FLAS LIFE SCIENCES, Seoul (KR)
Appl. No. 17/041,670
Filed by G+FLAS LIFE SCIENCES, Seoul (KR)
PCT Filed Mar. 27, 2019, PCT No. PCT/KR2019/003585
§ 371(c)(1), (2) Date Sep. 25, 2020,
PCT Pub. No. WO2019/190198, PCT Pub. Date Oct. 3, 2019.
Claims priority of application No. 10-2018-0035298 (KR), filed on Mar. 27, 2018.
Prior Publication US 2021/0128697 A1, May 6, 2021
Int. Cl. A61K 48/00 (2006.01); A61K 38/46 (2006.01); A61K 47/64 (2017.01); A61P 35/00 (2006.01); A61K 31/7105 (2006.01)
CPC A61K 38/465 (2013.01) [A61K 31/7105 (2013.01); A61K 47/64 (2017.08); A61P 35/00 (2018.01)] 6 Claims
 
1. A method of treating a cancer in a subject in need thereof comprising administering an effective amount of a composition to the subject,
wherein the composition comprises a vector containing (a) an isolated polynucleotide complementarily binding to a nucleic acid specifically present in cancer cells; (b) an isolated polynucleotide encoding an endonuclease; and (c) an isolated polynucleotide encoding an exonuclease, and
wherein the exonuclease is selected from the group consisting of RecBCD, RecE, RecJ, T5, Exo I, Exo III, Exo VII, Lexo, TREX2, Exoribonuclease T, TREXI, Mungbean exonuclease, Lambda, and a combination thereof,
wherein the nucleic acid specifically present in cancer cells is selected from the genes of which copy number variation (CNV) is at least 4,
wherein the endonuclease is a clustered regularly interspaced short palindromic repeat (CRISPR)-associated protein selected from a group consisting of Cas1, Cas1B, Cas2, Cas3, Cas4, Cas5, Cas6, Cas7, Cas8, Cas9, Cas10, Cas12a, Cas12b, Cas12c, Cas12d, Cas12e, Cas12g, Cas12h, Cas12i, Cas13a, Cas13b, Cas13c, Cas13d, Cas14, Csy1, Csy2, Csy3, Cse1, Cse2, Csc1, Csc2, Csa5, Csn2, CsMT2, Csm3, Csm4, Csm5, Csm6, Cmr1, Cmr3, Cmr4, Cmr5, Cmr6, Csb1, Csb2, Csb3, Csx17, Csx14, Csx10, Csx16, CsaX, Csx3, Csx1, Csx15, Csf1, Csf2, Csf3, and Csf4, and
wherein the endonuclease and the exonuclease are of a fusion protein.