	US 11,865,159 B2
	Therapeutic RNA
Friederike Gieseke, Mainz (DE); Ugur Sahin, Mainz (DE); Timothy R. Wagenaar, Sudbury, MA (US); Dmitri G. Wiederschain, Bridgewater, NJ (US); and Christian Hotz, Mainz (DE)
Assigned to Sanofi, Paris (FR)
Filed by Sanofi, Paris (FR)
Filed on Apr. 30, 2021, as Appl. No. 17/245,605.
Application 17/245,605 is a division of application No. 16/552,248, filed on Aug. 27, 2019.
Application 16/552,248 is a continuation of application No. PCT/US2018/019878, filed on Feb. 27, 2018.
Claims priority of provisional application 62/597,527, filed on Dec. 12, 2017.
Claims priority of provisional application 62/464,981, filed on Feb. 28, 2017.
Claims priority of application No. 17306089 (EP), filed on Aug. 23, 2017.
Prior Publication US 2021/0290730 A1, Sep. 23, 2021
Int. Cl. A61K 38/20 (2006.01); A61P 35/00 (2006.01); A61K 9/00 (2006.01); A61K 38/09 (2006.01); A61K 38/21 (2006.01); A61K 48/00 (2006.01); A61K 39/395 (2006.01); A61K 38/19 (2006.01)

CPC A61K 38/208 (2013.01) [A61K 9/0019 (2013.01); A61K 38/193 (2013.01); A61K 38/2086 (2013.01); A61K 38/212 (2013.01); A61K 39/3955 (2013.01); A61K 48/00 (2013.01); A61P 35/00 (2018.01)]

30 Claims

1. A composition comprising RNA encoding an IL-12sc protein, RNA encoding an IL-15 sushi protein, RNA encoding an IFNα protein, and RNA encoding a GM-CSF protein, wherein each RNA comprises a modified nucleoside in place of each uridine, wherein the modified nucleoside is pseudouridine (ψ), N1-methyl-pseudouridine (m1ψ), 5-methyl-uridine (m5U), or a combination thereof, and wherein

the IL-12sc protein comprises an amino acid sequence having at least 95% identity to the amino acid sequence of SEQ ID NO: 14;

the IL-15 sushi protein comprises an amino acid sequence having at least 95% identity to the amino acid sequence of SEQ ID NO: 24

the IFNα protein comprises an amino acid sequence having at least 95% identity to the amino acid sequence of SEQ ID NO: 19; and

the GM-CSF protein comprises an amino acid sequence having at least 95% identity to the amino acid sequence of SEQ ID NO: 27.