	US 11,865,083 B2
	Rapid method of generating live attenuated vaccines
Eng Eong Ooi, Singapore (SG); Kenneth Goh, Singapore (SG); Swee Sen Kwek, Singapore (SG); and Choon Kit Tang, Singapore (SG)
Assigned to NATIONAL UNIVERSITY OF SINGAPORE, Singapore (SG)
Filed by NATIONAL UNIVERSITY OF SINGAPORE, Singapore (SG)
Filed on Feb. 26, 2021, as Appl. No. 17/249,329.
Application 17/249,329 is a continuation of application No. 16/093,262, abandoned, previously published as PCT/SG2017/050211, filed on Apr. 13, 2017.
Claims priority of application No. 10201602980W (SG), filed on Apr. 14, 2016.
Prior Publication US 2021/0187094 A1, Jun. 24, 2021
Int. Cl. A61K 39/12 (2006.01); A61K 39/00 (2006.01)

CPC A61K 39/12 (2013.01) [A61K 2039/5254 (2013.01); C12N 2770/24121 (2013.01); C12N 2770/24122 (2013.01); C12N 2770/24134 (2013.01); C12N 2770/24164 (2013.01); Y02A 50/30 (2018.01)]

17 Claims

1. A vaccine comprising a nucleic acid sequence encoding the genome of a maladapted Zika virus, wherein the nucleic acid sequence comprises a sequence selected from the group consisting of SEQ NO: 39, SEQ ID NO: 40, SEQ ID NO: 41, and SEQ ID NO: 42.