CPC A01H 1/126 (2021.01) [A01H 1/045 (2021.01); A01H 5/08 (2013.01); A01H 6/344 (2018.05); C12Q 1/6895 (2013.01); C12Q 2600/13 (2013.01); C12Q 2600/156 (2013.01)] | 7 Claims |
1. A method for breeding Cucumis melo subsp. melo plants resistant to Tomato leaf curl New Delhi virus (ToLCNDV), comprising the steps of crossing a plant resistant to ToLCNDV comprising homozygously in its genome on chromosome 11 introgressed sequences from C. melo subsp. agrestis var. acidulous, conferring said resistance only when present homozygously; with a Cucumis melo subsp. melo plant susceptible or less resistant to ToLCNDV,
wherein said introgressed sequences on chromosome 11 conferring the resistance are present at least at two loci selected from the group consisting of the loci defined by the SNP markers Melon_sbg_617_42, the sequence of which is set forth in SEQ ID No:1, Melon_sbg_617_84, the sequence of which is set forth in SEQ ID No:2, Melon_sbg_20578_63, the sequence of which is set forth in SEQ ID No:3, Melon_sbg_20578_82, the sequence of which is set forth in SEQ ID No:4, Melon_sbg_55680_17, the sequence of which is set forth in SEQ ID No:5, Melon_sbg_60684_74, the sequence of which is set forth in SEQ ID No:6, Melon_sbg_33761_74, the sequence of which is set forth in SEQ ID No:7, SNP Melon_sbg_2720_78, the sequence of which is set forth in SEQ ID No:8, SNP Melon_sbg_14207_58, the sequence of which is set forth in SEQ ID No:9, SNP Melon_sbg_22016_27, the sequence of which is set forth in SEQ ID No:10, SNP Melon_sbg_22016_30, the sequence of which is set forth in SEQ ID No:11, Melon_sbg_22016_36, the sequence of which is set forth in SEQ ID No:12, Melon_sbg_11556_13, the sequence of which is set forth in SEQ ID No:13, Melon_sbg_24259_45, the sequence of which is set forth in SEQ ID No:14, Melon_sbg_16835_5, the sequence of which is set forth in SEQ ID No:15 and Melon_sbg_16835_17, the sequence of which is set forth in SEQ ID No:17, wherein the presence of the introgressed sequence at said loci are characterized by the presence of allele T of SNP Melon_sbg_33761_74 and/or the presence of allele G of SNP Melon_sbg_2720_78 and/or the presence of allele A of SNP Melon_sbg_14207_58 and/or the presence of allele G of SNP Melon_sbg_22016_27 and/or the presence of allele G of SNP Melon_sbg_22016_30 and/or the presence of allele G of SNP Melon_sbg_22016_36.
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