	US 11,860,166 B2
	Red fluorescent protein-based biosensor for measuring activity of dopamine receptor D1
Jihye Seong, Seoul (KR); and Hyun-bin Kim, Seoul (KR)
Assigned to KOREA INSTITUTE OF SCIENCE AND TECHNOLOGY, Seoul (KR)
Filed by KOREA INSTITUTE OF SCIENCE AND TECHNOLOGY, Seoul (KR)
Filed on Jan. 31, 2020, as Appl. No. 16/778,306.
Claims priority of application No. 10-2019-0120561 (KR), filed on Sep. 30, 2019.
Prior Publication US 2021/0096133 A1, Apr. 1, 2021
Int. Cl. G01N 33/58 (2006.01); C07K 14/435 (2006.01); C07K 14/705 (2006.01); G01N 33/94 (2006.01); C12N 15/85 (2006.01); C12N 15/62 (2006.01)

CPC G01N 33/582 (2013.01) [C07K 14/43504 (2013.01); C07K 14/70571 (2013.01); C12N 15/62 (2013.01); C12N 15/85 (2013.01); G01N 33/9413 (2013.01); C07K 2319/60 (2013.01)]

14 Claims

1. A red fluorescent protein-based biosensor for measuring the activity of dopamine receptor D1, the biosensor comprising:

a recombinant gene comprising a dopamine receptor D1 gene, and a gene encoding a modified circularly permuted red fluorescent protein (cpRFP) ;

a recombinant expression vector comprising the recombinant gene; or

a transformant transformed with the recombinant expression vector, wherein the gene encoding the modified circularly permuted red fluorescent protein (cpRFP) comprises a gene encoding an amino acid sequence of the following Formula 1:

L₁-cpRFP-L₂ [Formula 1]

wherein, L₁comprises a linker peptide having LSS and any 2 independently selected amino acid residues at the N-terminus of cpRFP, and L₂comprises a linker peptide having any 2 independently selected amino acid residues and DDL at the C-terminus of cpRFP.