US 11,860,071 B2
Lossless cryo-grid preparation by controlled sample evaporation
Thomas Braun, Munchenstein (CH); Stefan Arnold, Uerikon (CH); and Henning Stahlberg, Binningen (CH)
Assigned to UNIVERSITAT BASEL, Basel (CH)
Appl. No. 16/342,529
Filed by UNIVERSITAT BASEL, Basel (CH)
PCT Filed Oct. 17, 2017, PCT No. PCT/EP2017/076467
§ 371(c)(1), (2) Date Apr. 17, 2019,
PCT Pub. No. WO2018/073242, PCT Pub. Date Apr. 26, 2018.
Claims priority of application No. 16194230 (EP), filed on Oct. 17, 2016.
Prior Publication US 2019/0250078 A1, Aug. 15, 2019
Int. Cl. G01N 1/00 (2006.01); G01N 1/28 (2006.01); G01N 1/42 (2006.01); G01N 1/44 (2006.01)
CPC G01N 1/2813 (2013.01) [G01N 1/42 (2013.01); G01N 1/44 (2013.01)] 15 Claims
OG exemplary drawing
 
1. Method for preparing a sample on a support structure (20), wherein
the sample is provided as a thin film having a film thickness on a support structure (20);
the temperature of the support structure (20) is adjusted to a value above the dew point temperature of the environment, such that the film thickness decreases;
light is directed at the support structure (20);
at least one intensity value of the light transmitted by the support structure (20) is measured;
the support structure (20) is automatically inserted into a liquid cryogen (80) dependent on the at least one measured intensity value, such that the sample is cooled down to an amorphous solid.