	US 11,859,250 B1
	Methods for treating eosinophilic esophagitis
Marc E. Rothenberg, Cincinnati, OH (US); Tetsuo Shoda, Cincinnati, OH (US); and Ting Wen, Cincinnati, OH (US)
Assigned to CHILDREN'S HOSPITAL MEDICAL CENTER, Cincinnati, OH (US)
Filed by CHILDREN'S HOSPITAL MEDICAL CENTER, Cincinnati, OH (US)
Filed on Feb. 21, 2019, as Appl. No. 16/281,750.
Claims priority of provisional application 62/634,446, filed on Feb. 23, 2018.
Int. Cl. C12Q 1/68 (2018.01); C12Q 1/6883 (2018.01)

CPC C12Q 1/6883 (2013.01) [C12Q 2600/106 (2013.01); C12Q 2600/158 (2013.01)]

18 Claims

1. A method for treating eosinophilic esophagitis (EoE) in a subject determined to have a severe form of EoE, the method comprising

subjecting a biological sample from the subject to a method for gene expression analysis,

determining the expression of a panel of genes in the biological sample, the panel comprising ACPP, CITED2, CTNNAL1, EML1, FLG, GRPEL2, MT1M, PNLIPRP3, and TSPAN12,

determining the subject's EoE endotype is severe based on the expression of the panel of genes, wherein a decrease in expression of one or more of CTNNAL1, EML1, FLG, PNLIPRP3, and TSPAN12 by at least 2-fold compared to a reference indicates the subject's EoE endotype is severe,

and

treating the patient with an EoE therapy comprising one or more of esophageal dilation, anti-cytokine therapy, and glucocorticoid therapy.