US 11,859,239 B2
Nucleic acid sample-contained container, method and apparatus for producing nucleic acid sample-contained container, non-transitory recording medium storing program for producing nucleic acid sample-contained container, and nucleic acid sample
Yudai Kawashima, Kanagawa (JP); Masayuki Yumoto, Kanagawa (JP); Satoshi Izumi, Tokyo (JP); and Manabu Seo, Kanagawa (JP)
Assigned to Ricoh Company, Ltd., Tokyo (JP)
Filed by RICOH COMPANY, LTD., Tokyo (JP)
Filed on Mar. 18, 2019, as Appl. No. 16/356,591.
Claims priority of application No. 2018-051756 (JP), filed on Mar. 19, 2018; application No. 2018-051757 (JP), filed on Mar. 19, 2018; and application No. 2019-043914 (JP), filed on Mar. 11, 2019.
Prior Publication US 2019/0284611 A1, Sep. 19, 2019
Int. Cl. C12Q 1/6806 (2018.01); C12Q 1/6809 (2018.01); C12Q 1/68 (2018.01); B01L 3/00 (2006.01); B01L 7/00 (2006.01); B41M 5/00 (2006.01); G01N 21/64 (2006.01)
CPC C12Q 1/6806 (2013.01) [B01L 3/56 (2013.01); B01L 7/52 (2013.01); B41M 5/0023 (2013.01); C12Q 1/68 (2013.01); C12Q 1/6809 (2013.01); G01N 21/6428 (2013.01); G01N 2021/6439 (2013.01)] 15 Claims
OG exemplary drawing
 
1. A nucleic, acid sample-contained container, comprising:
a plurality of shapes selected from the group consisting of a concave bottom and a section on a substrate, wherein each of the plurality of shapes comprises a droplet consisting of:
a solution, wherein the solution is selected from the group consisting of water, nuclease-free water, a broth, a separation liquid, a diluted solution, a buffer, an organic substance lysing liquid, an organic solvent, an electrolyte aqueous solution, an inorganic salt aqueous solution, a metal aqueous solution, a labeling agent or reagent, and a mixture liquid of these solutions;
a nucleic acid amplifying agent and a fluorescent labeling reagent; and
at least one of a first nucleic acid molecule and a plurality of a second nucleic acid molecule, wherein the at least one of the first nucleic acid molecule and the plurality of the second nucleic acid molecule are unbound to a particle, and wherein the plurality of the second nucleic acid molecule corresponds to an amplified nucleic acid and is in excess relative to the at least one of the first nucleic acid molecule;
wherein each of the at least one of the first nucleic acid molecule comprises an intended base sequence and a base sequence for detection different from the intended base sequence, and wherein the intended base sequence is present in each of the plurality of shapes in a predetermined number that is less than 1,000;
wherein each of the plurality of the second nucleic acid molecule comprises the base sequence for detection and is free of the intended base sequence,
wherein a coefficient of variation (CV value) for the predetermined number in which the intended base sequence is comprised is lower than 20%.