	US 11,859,230 B2
	Compositions and methods for microbial co-culture
Jeremy Minty, Ann Arbor, MI (US); Marc E. Singer, Ann Arbor, MI (US); Xiaoxia Lin, Ann Arbor, MI (US); and David Boyer, Ann Arbor, MI (US)
Assigned to The Regents of the University of Michigan, Ann Arbor, MI (US)
Appl. No. 16/304,336
Filed by The Regents of the University of Michigan, Ann Arbor, MI (US)
PCT Filed May 26, 2017, PCT No. PCT/US2017/034696 § 371(c)(1), (2) Date Nov. 26, 2018, PCT Pub. No. WO2017/205750, PCT Pub. Date Nov. 30, 2017.
Claims priority of provisional application 62/341,984, filed on May 26, 2016.
Prior Publication US 2019/0136271 A1, May 9, 2019
Int. Cl. C12P 7/48 (2006.01); C12M 1/00 (2006.01); C12P 39/00 (2006.01); C12P 13/02 (2006.01)

CPC C12P 7/48 (2013.01) [C12M 23/58 (2013.01); C12P 13/02 (2013.01); C12P 39/00 (2013.01)]

12 Claims

1. A method of producing γ-polyglutamic acid (PGA), comprising:

a) contacting an Aspergillus niger, Yarrowia lipolytica, or Candida oleophila fungus with a first feedstock in a bioreactor;

b) fermenting said fungus to produce a culture broth comprising citric acid in the presence of a nitrogen source at a concentration of 0.1-50 g total N/L and a phosphorous source at a concentration of 0.1-25 g total P/L;

c) contacting said bioreactor comprising said culture broth comprising citric acid from step b), without purification, with a Bacillus sp., wherein said Bacillus sp. is Bacillus subtilis or Bacillus licheniformis and a second feedstock; and

d) fermenting said Bacillus sp. to generate PGA in the presence of a nitrogen source at a concentration of 0.1-50 g total N/L and a phosphorous source at a concentration of 0.1-25 g total P/L.