CPC C12N 9/1264 (2013.01) [C12Y 207/07031 (2013.01)] | 10 Claims |
1. A terminal deoxynucleotidyl transferase (TdT) variant comprising an amino acid sequence at least ninety percent identical to the amino acid sequence of SEQ ID NO: 3 with a substitution at positions Q326 and K265, wherein the TdT variant (i) is capable of synthesizing a nucleic acid fragment without a template and (ii) is capable of incorporating a 3′-O-modified nucleotide onto a nucleic acid fragment.
|