	US 11,859,210 B2
	Methods of differentiating stem cells into chondrocytes
Darryl D. D'Lima, San Diego, CA (US); Tsaiwei Olee, San Diego, CA (US); and Clifford W. Colwell, La Jolla, CA (US)
Assigned to SCRIPPS HEALTH, San Diego, CA (US)
Filed by Scripps Health, San Diego, CA (US)
Filed on Jun. 5, 2020, as Appl. No. 16/894,738.
Application 16/894,738 is a division of application No. 14/431,893, granted, now 10,724,005, previously published as PCT/US2013/062437, filed on Sep. 27, 2013.
Claims priority of provisional application 61/707,808, filed on Sep. 28, 2012.
Prior Publication US 2020/0299648 A1, Sep. 24, 2020
Int. Cl. C12N 5/077 (2010.01); A61K 35/32 (2015.01)

CPC C12N 5/0655 (2013.01) [A61K 35/32 (2013.01); C12N 2501/15 (2013.01); C12N 2506/02 (2013.01); C12N 2533/54 (2013.01); C12N 2533/90 (2013.01); C12N 2537/10 (2013.01)]

20 Claims

1. A method of regenerating cartilaginous tissue, the method comprising:

a. culturing pluripotent cells directly on tissue culture-grade plastic;

b. passaging and culturing the pluripotent cells at least two times to generate a population of cells expressing at least three proteins selected from CD90, CD44, CD105, and CD73;

c. culturing the population of cells expressing at least three proteins selected from CD90, CD44, CD105, and CD73 in a three-dimensional culture to generate chondrogenic precursor cells; and

d. transplanting the chondrogenic precursor cells into a bone or cartilage defect, wherein a regenerated cartilaginous tissue is produced in the bone or cartilage defect.