	US 11,859,205 B2
	Medium for culturing stem cells
Yoko Kuriyama, Kawasaki (JP); Sho Senda, Kawasaki (JP); Yumi Ando, Kawasaki (JP); Tomomi Yoshida, Kawasaki (JP); Haruna Sato, Kawasaki (JP); Daisuke Ejima, Kawasaki (JP); Takayoshi Fujii, Kawasaki (JP); Masayo Date, Kawasaki (JP); and Manabu Kitazawa, Kawasaki (JP)
Assigned to AJINOMOTO CO., INC., Tokyo (JP)
Filed by AJINOMOTO CO., INC., Tokyo (JP)
Filed on Jun. 11, 2020, as Appl. No. 16/898,844.
Application 16/898,844 is a division of application No. 14/953,132, filed on Nov. 27, 2015, abandoned.
Application 14/953,132 is a continuation of application No. PCT/JP2014/064497, filed on May 30, 2014.
Claims priority of application No. 2013-114285 (JP), filed on May 30, 2013.
Prior Publication US 2020/0370006 A1, Nov. 26, 2020
Int. Cl. C12N 5/074 (2010.01); C12N 5/0735 (2010.01); C12N 5/071 (2010.01); C12N 5/00 (2006.01)

CPC C12N 5/0602 (2013.01) [C12N 5/0018 (2013.01); C12N 5/0696 (2013.01); C12N 5/0606 (2013.01); C12N 2500/36 (2013.01); C12N 2501/998 (2013.01)]

6 Claims

1. A method of culturing for proliferation of a stem cell while maintaining an undifferentiated state, comprising:

cultivating the stem cell in a medium comprising an albumin which comprises a reduced amount of long chain fatty acid; and

maintaining a proportion of differentiated cells within 10% at most,

wherein:

said albumin is present in said medium in a concentration of 0.05 mg/mL to 10 mg/mL, based on the volume of said medium,

and wherein said long chain fatty acid is at least one selected from the group consisting of oleic acid, palmitic acid, stearic acid, linoleic acid, linolenic acid, and arachidonic acid.