US 11,859,202 B2
Multi-functional fluid flow device
James Lyons, York, PA (US); Christopher Ward, Baltimore, MD (US); and Joseph Stains, Laurel, MD (US)
Assigned to University of Maryland, Baltimore, Baltimore, MD (US)
Filed by University of Maryland, Baltimore, Baltimore, MD (US)
Filed on Jan. 21, 2021, as Appl. No. 17/154,239.
Application 17/154,239 is a continuation of application No. 15/466,255, filed on Mar. 22, 2017, granted, now 10,927,341.
Claims priority of provisional application 62/311,654, filed on Mar. 22, 2016.
Prior Publication US 2021/0214677 A1, Jul. 15, 2021
This patent is subject to a terminal disclaimer.
Int. Cl. B01L 3/00 (2006.01); C12N 5/00 (2006.01); G01N 33/543 (2006.01); C12M 1/32 (2006.01); C12M 1/42 (2006.01)
CPC C12N 5/00 (2013.01) [B01L 3/50853 (2013.01); C12M 23/12 (2013.01); C12M 35/04 (2013.01); G01N 33/54366 (2013.01); B01L 2200/026 (2013.01); B01L 2300/046 (2013.01); B01L 2300/0829 (2013.01)] 4 Claims
OG exemplary drawing
 
1. A method of applying fluid flow to biological cells in a culturing well, comprising:
positioning a fluid flow device in a culturing well of a multiwell cell culture plate, the device comprising an elongate tubular body having an upper proximal end, a lower distal end, and a length therebetween, the distal end of the elongate tubular body configured for insertion into a well of a multiwell cell culture plate; at least one first source port positioned on the distal end; at least one first waste port positioned on the distal end; at least one second source port positioned on the elongate tubular body above the at least one first source port; at least one second waste port positioned on the elongate tubular body above the at least one first waste port; at least one source conduit positioned within the body connecting a single first source port out of the at least one first source port(s) to a single second source port out of the at least one second source port(s); and at least one waste conduit positioned within the body connecting a single first waste port out of the at least one first waste port(s) to a single second waste port out of the at least one second waste port(s), such that the at least one first source port and the at least one first waste port are positioned within the well; and
flowing a fluid through the device;
wherein the fluid applies a shear stress to the cells in the culturing well.