| CPC C12N 15/8273 (2013.01) [C12N 15/8218 (2013.01)] | 9 Claims |
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1. A method of obtaining a genetically engineered Brassicaceae plant having increased drought tolerance, the method comprising:
(i) transforming at least one Brassicaceae plant cell with a recombinant nucleic acid construct to produce at least one transformed Brassicaceae plant cell;
(ii) obtaining at least one genetically engineered Brassicaceae plant from said at least one transformed Brassicaceae plant cell produced in step (i); and
(iii) selecting a genetically engineered Brassicaceae plant from said at least one genetically engineered Brassicaceae plant obtained in step (ii) that exhibits increased drought tolerance relative to a control Brassicaceae plant or plant cell of the same species and grown under the same conditions, wherein the control plant or plant cell is from a Brassicaceae plant that has not been transformed with the recombinant nucleic acid construct,
wherein the recombinant nucleic acid construct comprises an expression cassette encoding at least one inhibitory polynucleotide that targets an endogenous Kanghan gene in the genetically engineered Brassicaceae plant or in said at least one transformed Brassicaceae plant cell to reduce or eliminate expression of a Kanghan protein encoded by the endogenous Kanghan gene, wherein said at least one inhibitory polynucleotide is expressed in said selected genetically engineered Brassicaceae plant in step (iii),
and
wherein the Kanghan protein has at least 95% amino acid sequence identity to the amino acid sequence as set forth in SEQ ID NO: 85.
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