US 11,859,193 B2
Plants with modified traits
XueRong Zhou, Harrison (AU); Qing Liu, Giralang (AU); Anna El Tahchy, Moncrieff (AU); Srinivas Belide, Moncrieff (AU); Madeline Claire Mitchell, Turner (AU); Uday Kumar Divi, Moncrieff (AU); Thomas Vanhercke, Kaleen (AU); James Robertson Petrie, Goulburn (AU); Surinder Pal Singh, Downer (AU); and Allan Graham Green, Cremorne Point (AU)
Assigned to NUSEED GLOBAL INNOVATION LTD., Manchester (GB)
Appl. No. 16/329,939
Filed by Commonwealth Scientific and Industrial Research Organisation, Acton (AU)
PCT Filed Sep. 1, 2017, PCT No. PCT/AU2017/050948
§ 371(c)(1), (2) Date Mar. 1, 2019,
PCT Pub. No. WO2018/039740, PCT Pub. Date Mar. 8, 2018.
Claims priority of application No. 2016903541 (AU), filed on Sep. 2, 2016; application No. 2016903577 (AU), filed on Sep. 6, 2016; application No. 2016904611 (AU), filed on Nov. 11, 2016; application No. PCT/AU2017/050012 (WO), filed on Jan. 6, 2017; and application No. 2017902756 (AU), filed on Jul. 13, 2017.
Prior Publication US 2019/0185871 A1, Jun. 20, 2019
Int. Cl. C12N 15/82 (2006.01); C11B 1/10 (2006.01); A23K 10/12 (2016.01); A23K 10/30 (2016.01); C11C 3/00 (2006.01); C11B 1/04 (2006.01); C10L 1/02 (2006.01); C11B 1/06 (2006.01); C12Q 1/6895 (2018.01)
CPC C12N 15/8247 (2013.01) [A23K 10/12 (2016.05); A23K 10/30 (2016.05); C10L 1/026 (2013.01); C11B 1/04 (2013.01); C11B 1/06 (2013.01); C11B 1/10 (2013.01); C11C 3/003 (2013.01); C12N 15/8218 (2013.01); C12N 15/8251 (2013.01); C12Q 1/6895 (2013.01); C10L 2200/0476 (2013.01); C10L 2200/0484 (2013.01); C10L 2270/026 (2013.01); C10L 2290/04 (2013.01); C10L 2290/24 (2013.01); C10L 2290/26 (2013.01); C12Q 2600/13 (2013.01)] 36 Claims
 
1. A process for producing a feedstuff for an animal, the process comprising a step of:
(i) harvesting vegetative plant parts from a transgenic Sorghum species plant or a transgenic Zea mays plant, the vegetative plant parts being transgenic for at least (a) a first exogenous polynucleotide which encodes a plant Wrinkled 1 (WRI1) polypeptide operably linked to a heterologous promoter which is effective to direct expression of the first exogenous polynucleotide in the Sorghum species or Zea mays vegetative plant parts and increase the level of the WRI1 polypeptide relative to a corresponding wild type vegetative plant part, and (b) a second exogenous polynucleotide which encodes a plant diacylglycerol acyltransferase (DGAT) polypeptide operably linked to a heterologous promoter which is effective to direct expression of the second exogenous polynucleotide in the Sorghum species or Zea mays vegetative plant parts and increase the level of the DGAT polypeptide relative to the corresponding wild type vegetative plant part, whereby the vegetative plant parts comprise a total fatty acid (TFA) content which comprises fatty acids esterified in the form of triacylglycerols (TAG) and fatty acids in the form of lipids other than TAG, wherein the vegetative plant parts comprise a TFA content of between 6% and 20% (w/w dry weight) and a TAG/TFA Quotient (TTQ) of between 0.60 and 0.84, or between 0.84 and 0.95, and one or more of the following steps:
(ii) admixing the harvested plant parts with at least one other feed ingredient,
(iii) baling the harvested plant parts,
(iv) processing the harvested plant parts by chopping, cutting, drying, pressing or pelleting the plant parts, and
(v) storing the harvested plant parts under conditions of reduced oxygen for a period of time until at least some carbohydrates in the plant parts are fermented to organic acids.