US 11,859,167 B2
Electrochemical measurement device and transducer
Ryota Kunikata, Tokyo (JP); Hiroyuki Hayashi, Tokyo (JP); Atsushi Suda, Tokyo (JP); Kosuke Ino, Miyagi (JP); Kumi Inoue, Miyagi (JP); and Tomokazu Matsue, Miyagi (JP)
Assigned to JAPAN AVIATION ELECTRONICS INDUSTRY, LIMITED, Tokyo (JP); and TOHOKU UNIVERSITY, Miyagi (JP)
Filed by JAPAN AVIATION ELECTRONICS INDUSTRY, LIMITED, Tokyo (JP); and TOHOKU UNIVERSITY, Miyagi (JP)
Filed on Jul. 2, 2021, as Appl. No. 17/366,945.
Application 17/366,945 is a continuation of application No. 16/336,782, granted, now 11,162,064, previously published as PCT/JP2017/033898, filed on Sep. 20, 2017.
Claims priority of application No. 2016-197818 (JP), filed on Oct. 6, 2016.
Prior Publication US 2021/0332321 A1, Oct. 28, 2021
Int. Cl. C12M 1/34 (2006.01); G01N 27/327 (2006.01); G01N 33/543 (2006.01); G01N 33/487 (2006.01); C12M 1/00 (2006.01)
CPC C12M 41/46 (2013.01) [C12M 1/00 (2013.01); C12M 1/34 (2013.01); C12M 41/32 (2013.01); G01N 27/3277 (2013.01); G01N 33/48728 (2013.01); G01N 33/5438 (2013.01)] 10 Claims
OG exemplary drawing
 
1. An electrochemical measurement device comprising a plurality of working electrodes, each of the plurality of working electrodes including an electrode surface supplying or receiving electrons to or from a chemical substance generated or consumed in a biological sample in a solution to cause an oxidation-reduction reaction, wherein
all of the electrode surfaces have diameter dimensions del not more than 80 μm and are arrayed on one flat surface in a configuration in which a plurality of electrode rows are arranged in a second direction orthogonal to a first direction, each of the plurality of electrode rows having the plurality of the electrode surfaces which are arranged in a straight line in the first direction,
a spacer is provided in a solution well containing the solution and the biological sample, the spacer having a profile surface at a distance h1 in a direction perpendicular to the one flat surface, the distance h1 satisfying
h1=21.8(del+0.8)/(del+9.7)±5[μm],
the spacer inhibiting the biological sample from entering a region on a side with the one flat surface of the profile surface and allowing a dissolved substance in the solution to diffuse, and
a first wall plate impervious to the dissolved substance in the solution is provided between two of the electrode rows adjacent to each other, the first wall plate extending in the first direction alongside two or more electrode surfaces arranged in one of said two of the electrode rows, having a height not less than a height of the spacer from the one flat surface such that the first wall plate reduces crosstalk in the second direction between said two of the electrode rows but the dissolved substance is not blocked from diffusing in the first direction in each of said two of the electrode rows.