US 11,859,162 B2
Method and apparatus for high throughput high efficiency transfection of cells
Vishal Tandon, Somerville, MA (US); Charles A. Lissandrello, Newtonville, MA (US); Jenna L. Balestrini, Boston, MA (US); Jonathan R. Coppeta, Windham, NH (US); and Patricia A. Swierk, Cambridge, MA (US)
Assigned to The Charles Stark Draper Laboratory, Inc., Cambridge, MA (US)
Filed by The Charles Stark Draper Laboratory, Inc., Cambridge, MA (US)
Filed on Aug. 30, 2019, as Appl. No. 16/557,820.
Claims priority of provisional application 62/725,382, filed on Aug. 31, 2018.
Prior Publication US 2020/0071727 A1, Mar. 5, 2020
Int. Cl. C12M 1/42 (2006.01); C12N 15/00 (2006.01); C12M 3/06 (2006.01); B01L 3/00 (2006.01); C12N 15/87 (2006.01)
CPC C12M 23/16 (2013.01) [C12M 35/02 (2013.01); B01L 3/50273 (2013.01); B01L 3/502715 (2013.01); B01L 2300/047 (2013.01); B01L 2300/087 (2013.01); B01L 2300/0864 (2013.01); B01L 2300/0887 (2013.01); B01L 2400/0496 (2013.01); C12N 15/87 (2013.01)] 18 Claims
OG exemplary drawing
 
1. A method for introducing a payload into cells, the method comprising:
directing cells from a first incubator to an assembly that contains multiple microfluidic devices;
acoustically driving cells from a cell culture medium flowing in a side stream of a sheath flow configuration, to an electroporation medium flowing through a central stream of the sheath flow configuration;
applying an electric field to cells in the electroporation medium; and
transferring or allowing the transfer of the payload into the cells.