CPC C12M 23/02 (2013.01) [C12M 23/40 (2013.01); C12M 23/44 (2013.01); C12M 23/58 (2013.01); C12M 25/06 (2013.01); C12M 27/00 (2013.01); C12M 27/02 (2013.01); C12M 27/16 (2013.01); C12M 29/10 (2013.01); C12M 29/16 (2013.01); C12M 29/18 (2013.01); C12M 33/14 (2013.01); C12M 41/12 (2013.01); C12M 41/26 (2013.01); C12M 41/44 (2013.01)] | 13 Claims |
1. A method of carrying out a bioreaction in a multivessel bioreactor system, the method comprising:
flowing a culture medium at an initial flow rate through fluidic paths between a master vessel and two or more slave vessels, each of the slave vessels being fluidically coupled in parallel to the master vessel by each of the slave vessels having a separate one of the fluidic paths extending directly from the slave vessel to the master vessel;
detecting a first culture medium parameter value in the master vessel or the slave vessels with a master sensor coupled to the master vessel;
transmitting the first culture medium parameter value from the master sensor to a control system in electronic communication with the master sensor;
determining an adjusted flow rate of the culture medium at the control system based on the first culture medium parameter value; and
controlling a bidirectional fluid transfer device with the control system to adjust the flow rate of the culture medium through the fluidic paths and maintain a substantially uniform value of the first culture medium parameter value in the master vessel or the slave vessels.
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