US 11,859,161 B2
Method of carrying out a bioreaction with multiple coupled vessels
Barbara Paldus, Woodside, CA (US); and Mark Selker, Los Altos Hills, CA (US)
Assigned to Finesse Solutions, Inc., Santa Clara, CA (US)
Filed by Finesse Solutions, Inc., Santa Clara, CA (US)
Filed on Feb. 22, 2022, as Appl. No. 17/677,517.
Application 17/677,517 is a division of application No. 15/427,613, filed on Feb. 8, 2017, granted, now 11,292,999.
Claims priority of provisional application 62/381,283, filed on Aug. 30, 2016.
Prior Publication US 2022/0169964 A1, Jun. 2, 2022
Int. Cl. C12M 1/00 (2006.01); C12M 3/00 (2006.01); B01L 3/00 (2006.01); C12M 1/02 (2006.01); C12M 1/34 (2006.01); C12M 1/06 (2006.01); C12M 3/06 (2006.01); C12M 1/12 (2006.01); C12M 1/26 (2006.01)
CPC C12M 23/02 (2013.01) [C12M 23/40 (2013.01); C12M 23/44 (2013.01); C12M 23/58 (2013.01); C12M 25/06 (2013.01); C12M 27/00 (2013.01); C12M 27/02 (2013.01); C12M 27/16 (2013.01); C12M 29/10 (2013.01); C12M 29/16 (2013.01); C12M 29/18 (2013.01); C12M 33/14 (2013.01); C12M 41/12 (2013.01); C12M 41/26 (2013.01); C12M 41/44 (2013.01)] 13 Claims
OG exemplary drawing
 
1. A method of carrying out a bioreaction in a multivessel bioreactor system, the method comprising:
flowing a culture medium at an initial flow rate through fluidic paths between a master vessel and two or more slave vessels, each of the slave vessels being fluidically coupled in parallel to the master vessel by each of the slave vessels having a separate one of the fluidic paths extending directly from the slave vessel to the master vessel;
detecting a first culture medium parameter value in the master vessel or the slave vessels with a master sensor coupled to the master vessel;
transmitting the first culture medium parameter value from the master sensor to a control system in electronic communication with the master sensor;
determining an adjusted flow rate of the culture medium at the control system based on the first culture medium parameter value; and
controlling a bidirectional fluid transfer device with the control system to adjust the flow rate of the culture medium through the fluidic paths and maintain a substantially uniform value of the first culture medium parameter value in the master vessel or the slave vessels.