CPC C07K 16/30 (2013.01) [A61K 39/39591 (2013.01); C07K 16/28 (2013.01); A61K 2039/505 (2013.01); C07K 2317/24 (2013.01); C07K 2317/33 (2013.01); C07K 2317/34 (2013.01); C07K 2317/565 (2013.01); C07K 2317/567 (2013.01); C07K 2317/73 (2013.01); C07K 2317/732 (2013.01); C07K 2317/734 (2013.01); C07K 2317/76 (2013.01); C07K 2317/92 (2013.01)] | 12 Claims |
1. A method of producing a transformed cell, the method comprising the steps of:
(a) transforming a human host cell with one or more expression vectors, wherein the one or more expression vectors comprise:
(i) a nucleic acid sequence encoding a first polypeptide comprising the antibody heavy chain CDR1, CDR2, and CDR3 regions having the amino acid sequences of positions 26-33, positions 51-58, and positions 97-106 of SEQ ID NO: 36, respectively; and
(ii) a nucleic acid sequence encoding a second polypeptide comprising the antibody light chain CDR1, CDR2, and CDR3 regions having the amino acid sequences of positions 27-31, positions 49-51, and positions 88-97 of SEQ ID NO: 35, respectively;
(b) culturing the transformed human host cell under conditions in which the transformed human host cell expresses the first and second polypeptides encoded by the one or more expression vectors; and
(c) harvesting the transformed human host cell expressing the first and second polypeptides.
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